动物医学进展Issue(11)：34-39,6.
以 Balb／c 小鼠为动物模型的 PCV-2疫苗效力检验方法的建立
Establishment of Test Method of Porcine Circovirus Type 2 Vaccine Potency in Animal Model of Balb/c Mice
摘要
Abstract
The animal model of Balb/c mice was established for PCV-2 vaccine immune efficacy in the study. The SPF female Balb/c mice of 4 week old were selected and randomly divided into 4 groups.The mice in-group Ⅰ were immunized with PCV-2 vaccine (0.1 mL/rat)by subcutaneous 1 point.The mice in groupⅡ were immunized with PCV-2 vaccine (0.2 mL/rat)by subcutaneous 2 points.The mice in group Ⅲ were immunized with PCV-2 vaccine (0.2 mL/rat)by subcutaneous 2 points and leg muscles.The mice in groupⅣ were not immunized with PCV-2 vaccine as the blank control group.The mice in immunized groups were immunized secondly in 7,14 and 21 d after first immunity.The blood in mice were took and the sera were separated after 7,10,14,21,28,35,42 days.The PCV-2 specific antibody was detected by ELISA in order to determine the best immune way and immune dose.The 4 week old female SPF Balb/c mice were selected and randomly divided into immune group,challenge control group and healthy control group.The mice in immunized and challenge control groups were attacked challenged by PCV-2 virulent strain after immunization 14 days.The differences of the mice in test groups were compared in clinical symptoms, pathological lesions,relative daily weight gain,PCV-2 DNA load and other aspects in order to determine the detection indexes of the animal model of mice.Mouse ELISA antibody test showed that the best im-mune procedure was subcutaneous and leg muscleinjection (0.2 mL/rat).The peak antibody value ap-peared 35 d after immunization,and was significantly higher than that of group I and II.The control mice showed weight loss,hair messy,mental tension and clinical symptoms,lymph node swelling,bleeding, pulmonary hemorrhage and other pathological lesion after challenge and the relative daily weight gain de-creased significantly.The PCV-2 DNA load difference was more than 103 .This study established the test method for PCV-2 vaccine potency in Balb/c mice as animal model.The dose and route of immunization were optimized,and the detection index and the corresponding technical parameters were determined, which laid a technical support for PCV-2 vaccine potency test.关键词
Balb/c小鼠/动物模型/猪圆环病毒2 型/疫苗效力Key words
Balb/c mouse/animal model/Porcine circovirus type 2/vaccine efficacy分类
农业科技引用本文复制引用
董林,王艳萍,谢金文,沈志强..以 Balb／c 小鼠为动物模型的 PCV-2疫苗效力检验方法的建立[J].动物医学进展,2014,(11):34-39,6.基金项目
山东省自然科学基金资助项目 （）
