动物医学进展Issue(11)：119-122,4.
氧化苦参碱-黄芪多糖复方对新城疫Ⅳ系疫苗免疫雏鸡血清IL-2和 IFN-γ水平的影响
Effects of Oxymatrine-Astragalus Polysaccharide Compound on Levels of IL-2 and IFN-γin Chicks Vaccinated with NDV Ⅳ Vaccine
李巨银 1胡新岗 1蔡丙严 1魏宁 1程汉 1桂文龙 1陈光明1
作者信息
- 1. 江苏农牧科技职业学院，江苏泰州 225300
- 折叠
摘要
Abstract
In order to evaluate the efficacy of oxymatrine-Astragalus polysaccharide(OTM-APS)against Newcastle disease virus (NDV)infection,The prevention efficacy of compound on Newcastle disease was evaluated.One hundred and fifty 14 days old Roman cocks without vaccination were randomly divided into 5 groups.the chickens in groups 1,2 and 3 were first intranasally administrated by NDV-Ⅳ vaccine.At the same time,the cocks in groups 1,2 and 3 were orally administrated with OTM-APS compounds of high,middle and low doses,respectively.Group 4 was vaccine control and Group 5 was blank control. Cocks in group 4 was vaccinated by vaccine and taken physiological saline,once a day for three days.The cocks were boost vaccinated at 28 days old.The concentrations of IL-2 and IFN-γin the sera of cocks in the 5 groups were detected by double antibody sandwich ELISA at 14,28 and 42 days old.The results showed that the concentrations of IL-2 and IFN-γin sera of the cocks treated with medicine were significantly high-er than that of the cocks in the vaccine control group (P <0.05).It indicated that OTM-APS compound could significantly strengthen the immune response of chickens to Newcastle disease vaccine and enhance the resistance against the disease..关键词
氧化苦参碱-黄芪多糖复方/公雏鸡/白细胞介素-2/干扰素/双抗夹心ELISAKey words
oxymatrine-Astragalus polysaccharide compound/Newcastle disease/IL-2/IFN-γ/double anti-body sandwich ELISA分类
农业科技引用本文复制引用
李巨银,胡新岗,蔡丙严,魏宁,程汉,桂文龙,陈光明..氧化苦参碱-黄芪多糖复方对新城疫Ⅳ系疫苗免疫雏鸡血清IL-2和 IFN-γ水平的影响[J].动物医学进展,2014,(11):119-122,4.
