动物医学进展Issue(12)：10-13,4.
山羊支原体山羊肺炎亚种和多杀性巴氏杆菌双重 PCR 检测方法的建立
Development of A Duplex PCR Assay for Detecting Mycoplasma capricolum subsp.capripneumoniae and Pasteurella multocida
摘要
Abstract
The purpose of this study was to establish a duplex PCR method for simultaneous detection of Mycoplasma capricolum subsp.capripneumoniae (Mccp)and Pasteurella multocida .Two sets of primers specific to Mccp and P .multocida were applied for development of the duplex PCR.After optimization of PCR components and reaction profile,a duplex PCR method was established.The results demonstrated that the assay is highly specific to Mccp and P .multocida ,and no-target pathogens were not detected. The detection limits of the assay were determined to be 32 pg for Mccp DNA and 50 pg for P .multocida DNA,respectively,which was as sensitive as single PCR methods.The developed duplex PCR could de-tect both of the pathogens from clinical samples.The duplex PCR established in this study will be useful for clinical detection,identification and epidemiological investigation of Mccp and P .multocida.关键词
山羊支原体山羊肺炎亚种/多杀性巴氏杆菌/双重PCRKey words
Mycoplasma capricolum subsp.capripneumoniae/Pasteurella multocida/duplex PCR分类
农业科技引用本文复制引用
亢宁宁,刀筱芳,冯旭飞,虎啸,马晓楠,杨发龙..山羊支原体山羊肺炎亚种和多杀性巴氏杆菌双重 PCR 检测方法的建立[J].动物医学进展,2014,(12):10-13,4.基金项目
西南民族大学大学生创新创业训练计划 （）
