动物医学进展Issue(12)：45-48,4.
羊口疮病毒威海株的分离鉴定
Isolation and Identification of Orf Virus Weihai Strain
摘要
Abstract
The virus strain was isolated from crust materials of lambs with Orf like-clinical symptoms from a goat farm in Weihai city of Shandong province by passage in primary bovine testicular cells (PBTC).The virus was identified as Orf virus using cell morphology of lesions,TCID50 test and PCR method.The re-sults showed that PBTC cultures infected with crust materials displayed cytopathic effects at the fifth pas-sages.The titer of the sixth passage virus was 106.5 TCID50/mL.The specific Orf virus B2L gene was am-plified.The product was sequenced,and the isolated strain was closest to similarity of nucleotide sequence (99%)with those of Fujian strain (KC588399.1,KC568398.1).It was concluded that the Orf virus strain was successfully isolated and identified.关键词
羊口疮病毒/分离鉴定/聚合酶链反应Key words
Orf virus/isolation and identification/PCR分类
农业科技引用本文复制引用
高晶晖,张瑜,侯伟杰,张立强,高洋,张淼涛,陈德坤..羊口疮病毒威海株的分离鉴定[J].动物医学进展,2014,(12):45-48,4.基金项目
陕西省科技统筹创新工程计划项目 （）
