动物医学进展Issue(12)：61-64,4.
重组 yEGFP 发光酵母细胞检测牛尿中雌激素类药物
Detection of Estrogen Like Drugs in Bovine Urine Based on Recombinant yEGFP Fluorescent Yeast Cells
摘要
Abstract
This paper was conducted to bioassay estrogen-like drug residues in urine of adult cows and calves based on the recombinant human estrogen receptor (hER)gene yeast cells (W303-1A/hER-ERE-yEGFP), respectively.This recombinant yeast cells is a fluorescent yeast cell,whose fluorescent density has a dose effect relationship with estrogenic compounds.The results showed that although the estrogenic compounds (1.000×10-4 estradiol equivalent)in adult cow urine samples was higher than that (2.518×10 -5 estradiol equivalent)in calf,but statistical significant difference was not found.The procedure provides a efficient, rapid,high-throughput method for routine residual analysis of estrogen like drugs in bovine urine.关键词
17β-雌二醇/重组基因yEGFP发光酵母细胞/牛尿Key words
17β-estradiol/recombinant gene yEGFP light yeast cell/bovine urine分类
农业科技引用本文复制引用
达剑森,李湘鸣,刘宗平..重组 yEGFP 发光酵母细胞检测牛尿中雌激素类药物[J].动物医学进展,2014,(12):61-64,4.基金项目
江苏省扬州市科技攻关项目 （）
