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复方苦豆子灌注液总生物碱含量测定方法的建立

辛任升 刘宇 张超 梁剑平

动物医学进展Issue(12):89-91,92,4.
动物医学进展Issue(12):89-91,92,4.

复方苦豆子灌注液总生物碱含量测定方法的建立

Establishment of Method for Determination of Total Alkaloids in Compound Sophora alopecuroides Perfusion

辛任升 1刘宇 2张超 2梁剑平1

作者信息

  • 1. 甘肃农业大学动物医学院,甘肃兰州 730070
  • 2. 中国农业科学院兰州畜牧与兽药研究所,农业部兽用药物创制重点实验室,甘肃省新兽药工程重点实验室,甘肃兰州 730050
  • 折叠

摘要

Abstract

To establish a method for the determination of total alkaloids in Compound Sophora Alopecui-oides Perfusate,oxymatrine as the reference,the total alkaloids were determined by acid dye colorimetry at 420 nm.The results showed that the linear ranges of the oxymatrine were 2.0 μg/mL-10 μg/mL.The re-gression equation was A=0.005 8C+0.111 6,(r=0.999 2).The average recovery rate was 99.6% (RSD=2.20%).The method was simple,accurate and reproducible.

关键词

复方苦豆子灌注液/酸性染料比色法/总生物碱/氧化苦参碱

Key words

compound Sophora alopecuioides perfusate/acid dye colorimetry/total alkaloid/oxymatrine

分类

农业科技

引用本文复制引用

辛任升,刘宇,张超,梁剑平..复方苦豆子灌注液总生物碱含量测定方法的建立[J].动物医学进展,2014,(12):89-91,92,4.

基金项目

“十二五”农村领域国家科技计划项目 ()

动物医学进展

OA北大核心CSTPCD

1007-5038

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