动物医学进展Issue(12):89-91,92,4.
复方苦豆子灌注液总生物碱含量测定方法的建立
Establishment of Method for Determination of Total Alkaloids in Compound Sophora alopecuroides Perfusion
摘要
Abstract
To establish a method for the determination of total alkaloids in Compound Sophora Alopecui-oides Perfusate,oxymatrine as the reference,the total alkaloids were determined by acid dye colorimetry at 420 nm.The results showed that the linear ranges of the oxymatrine were 2.0 μg/mL-10 μg/mL.The re-gression equation was A=0.005 8C+0.111 6,(r=0.999 2).The average recovery rate was 99.6% (RSD=2.20%).The method was simple,accurate and reproducible.关键词
复方苦豆子灌注液/酸性染料比色法/总生物碱/氧化苦参碱Key words
compound Sophora alopecuioides perfusate/acid dye colorimetry/total alkaloid/oxymatrine分类
农业科技引用本文复制引用
辛任升,刘宇,张超,梁剑平..复方苦豆子灌注液总生物碱含量测定方法的建立[J].动物医学进展,2014,(12):89-91,92,4.基金项目
“十二五”农村领域国家科技计划项目 ()