动物医学进展Issue(1)：19-22,4.
犬细小病毒 JL-M13株的分离鉴定及全基因组序列分析
Isolation,Identification and Sequence Analysis of Complete Genome of Canine Parvovirus JL-M13 Strain
摘要
Abstract
We inoculated canine parvovirus positive samples into F 81 cells and isolated a virus strain named JL‐M13 by three passages .Five nucleotide sequences were amplified from the DNA extracted from canine parvovirus wild strain by overlapping PCR .Splicing and analysis of the sequences showed that the genome length of the strain were 4621bp ,the identity with other canine parvovirus was 98 .2%‐99 .4% .Phyloge‐netic tree analysis showed that the similarity rate of JL‐M13 with CPV‐2 (EF011664 .1)was the highest and was significantly lower with canine parvovirus type‐2 vaccine strain .关键词
犬细小病毒/分离/全基因组/序列分析Key words
Canine parvovirus/isolation/complete genome/sequence analysis分类
农业科技引用本文复制引用
仝明薇,易立,程世鹏..犬细小病毒 JL-M13株的分离鉴定及全基因组序列分析[J].动物医学进展,2015,(1):19-22,4.基金项目
吉林省科技发展项目（20140204066 N Y ）；吉林省重大科技成果转化项目（10ZDZ H010）；吉林市科技发展资助项目 （）
