动物医学进展Issue(1)：40-44,5.
鸡传染性鼻炎和新城疫二联灭活疫苗的研究
Studies on Combined Infectious Coryza and Newcastle Disease Inactivated Vaccines
摘要
Abstract
A combined infectious coryza and newcastle disease inactivated vaccine was developed using page's serovar A ,B and C of Avibacterium paragallinarum (Avpg) and La Sota strain of NDV .Three batch of vaccines were used for safety test ,The Chicken were injected 0 .5 mL/chicken at a time for three times and 2 .0 mL/chicken at a time .We carry out vaccine efficacy experiment with three batches of vaccines for SPF chicken ,30 days after immunization ,the chickens were challenged with Page's serovar A ,B and C of Avpg .The immune duration test of the conventional chickens which were immunized at 42 days‐old and 110 days‐old .Nine months after second immunization ,the chickens were challenged with Page's A ,B ,C of Avpg and virulent Newcastle Disease virus .The effect of storage on the vaccine was evaluated in SPF chickens using three batches of vaccine stored at 4 ℃‐8 ℃ for 12 and 15 months .The result show that the vaccines is very safe ,and challenged protection rate of immunized SPF chickens is ≥ 80% for Page's sero‐var A ,B and C of Avpg .HI titer of ND is great than 24 for immunized SPF chickens using 20 μL vaccine . The conventional chickens were challenged at 9 months post second vaccination and the protection rate ≥70% for all three serovars ,and 100% against the virulent Newcastle Disease virus .The protection rate of immunized chickens using the vaccine stored at 4 ℃‐8 ℃ for 12 and 15 months is ≥70% for Page's serovar A ,B and C of Avpg .,HI titer is great than 24 and 7/10 against the virulent Newcastle Disease virus .The vaccine is safe and effective .关键词
鸡传染性鼻炎/新城疫/三价/二联/灭活疫苗Key words
Infectious coryza/Newcastle disease/Avibacterium paragallinarum/trivalence combined/inac-tivited vaccine分类
农业科技引用本文复制引用
龚玉梅,张培君,朱文革,贺云霞,王宏俊,张瑜,邵庆红..鸡传染性鼻炎和新城疫二联灭活疫苗的研究[J].动物医学进展,2015,(1):40-44,5.基金项目
公益性行业（农业）科研专项经费项目（201303044）；北京市财政专项资助项目 （）
