动物医学进展Issue(1)：45-48,4.
三种佐剂对奶山羊乳房炎灭活疫苗免疫效果的影响
Effects of Three Adjuvants on Immune Effect of Dairy Goat Mastitis Inactivated Vaccine
摘要
Abstract
In order to select an effective adjuvant for inactivated vaccine against dairy goat mastitis ,E .coli , Staphylococcus aureus ,coagulase negative Staphylococcus epidermidis and Staphylococcus chromogenes were inactivated by using methanal at 37 ℃ and then thoroughly mixed with aluminium salt ,propolis or transfer factor to prepare aluminium salt ,propolis or transfer factor adjuvant vaccines .Each lactating dair‐y goat was inoculated subcutaneously via neck twice with a interval of 14 d and the dosage was 2 mL each time .Blood and milk samples were collected before and 10 days after each inoculation .The levels of anti‐bodies were measured by an indirect ELISA while the numbers of goats suffering subclinical mastitis were measured by a rapid diagnostic reagent .The results showed that antibodies against E .coli ,Staphylococcus aureus ,coagulase negative Staphylococcus epidermidis and Staphylococcus chromogenes in blood and milk of aluminium group were significantly higher than that of control group (P<0 .05) ,and aluminum group's the antibody levels were highest with the transfer factor group and propolis group followed .Aluminum ad‐juvant vaccine can significantly reduce the detection rate of subclinical mastitis ,and the rate was dropped from 60% before the immunization to 30% after the second injection .In conclusion ,compared with propo‐lis and transfer factor ,aluminum adjuvant can stimulate the body to produce stronger humoral immune re‐sponse and has a significant therapeutic effect to subclinical mastitis .关键词
乳房炎/灭活菌苗/佐剂/抗体效价/隐性乳房炎Key words
mastitis/inactivated vaccine/adjuvant/antibody level/subclinical mastitis分类
农业科技引用本文复制引用
侯伟杰,高洋,高晶晖,安贝,张淼涛,陈德坤..三种佐剂对奶山羊乳房炎灭活疫苗免疫效果的影响[J].动物医学进展,2015,(1):45-48,4.基金项目
陕西省科技统筹创新工程计划项目 （）
