动物医学进展Issue(1)：49-52,4.
三株 BHK-21悬浮细胞培养特性比较
Comparison of Culture Characteristics of Three BHK-21 Suspension Cell Strains
摘要
Abstract
In order to select the best cell line adapted to the productionof FMD vaccine ,the research com‐pared the culture characteristics of three kinds of different strains from BHK‐21 suspension cells under the same conditions with GMEM culture medium ,30 mL/L serum concentration .The results showed that ,no obvious difference between these three kinds of cells in morphology ,the cells can not be easily distinguish‐ed under the microscope .In the process of cultivation ,the BHK21‐L cell line can reach the maximum den‐sity in the shortest time ,the growth rate and cell activity is high ,DNA synthesis is exuberant .The maxi‐mum density of BHK21C13‐2P cell line is lower ,in the 72 h‐96 h was completely blocked in G0/G1 ,ampli‐fying smaller proportion ,1∶1 .5‐1∶2 ;BHK21‐B cells stopped proliferation in 72 h‐96 h ,can enlarge a larger proportion ,but be prone to cell cluster ,easy adherent phenomenon ,affect cell growth .Therefore , BHK21‐L cell is the best cell line producing cells for foot‐and‐mouth disease vaccine .关键词
BHK-21细胞/细胞形态/培养特性Key words
BHK-21 cell/cell morphology/culture characteristic分类
生物科学引用本文复制引用
武发菊,尚勇良,孙玉霞,董文教,安芳兰,万玉林,刘学荣,杨进才..三株 BHK-21悬浮细胞培养特性比较[J].动物医学进展,2015,(1):49-52,4.基金项目
甘肃省科技支撑计划项目 （）
