动物医学进展Issue(1)：61-65,5.
杨凌某羊场羊口疮病毒的分离鉴定
Isolation and Identification of Orf Virus in a Goat Farm of Yangling
摘要
Abstract
In order to obtain a wild strain of orf virus (ORFV ) in Yangling ,the lip scab of a lamb with typi‐cal orf symptoms from a goat farm in Yangling was collected .The scab was grinded and centrifuged suffi‐ciently after dipped in PBS overnight at 4 ℃ ,and then the supernatant was sterilized with 0 .22 μm filter and inoculated in the calf testis primary cells with 500μL .After 4 generations of blind passages ,the virus titer of the 5th generation was determined and a PCR assay based on B2L and F1L genes was used to detect the ORFV .The results suggested that the 5th generation of ORFV can cause apparent CPE in calf testis primary cells and the virus titer of the 5th generation was 107 .66 TCID50/mL .The fragments of B2L and F1L amplified by PCR were about 540 bp and 437 bp respectively ,and the similarities with its reference se‐quences are 96 .57% and 96 .43% .In conclusion ,a wild strain of ORFV in Yangling was isolated in this study .关键词
羊口疮病毒/PCR检测/犊牛睾丸原代细胞/分离/鉴定Key words
Orf virus/PCR detection/calf testis primary cell/isolation/identification分类
农业科技引用本文复制引用
张瑜,高晶晖,张立强,高洋,贾云晓,陈德坤..杨凌某羊场羊口疮病毒的分离鉴定[J].动物医学进展,2015,(1):61-65,5.基金项目
陕西省科技统筹创新工程计划项目 （）
