动物医学进展Issue(2)：29-33,5.
淮南麻黄鸡 MHC Ⅰα链基因的克隆及序列分析
cDNA Cloning and Sequence Analysis of MHC ClassⅠαGene in Huainan Partridge Chicken
摘要
Abstract
Typical major histocompatibility complex (MHC) is the key of antigen -presenting molecules , they play a crucial role in the immune system ,w hich closely linked related to many diseases .In order to further understand molecular characteristics of chicken M HC Class Ⅰ gene ,and peptide-binding regions , the 29 sequence of chicken M HC Ⅰαgene from huainai partridge chicken was cloned by RT‐PCR .The re‐sults revealed that M HC gene was highly polymorphic ,but they were mainly located in the α1 and α2 re‐gions .There were 68 of mutations ,which were distributed in the α1 andα2 regions ,but 22 of them were highly mutated sites . The amino acid homology among α1 and α2 regions of chicken M HC Ⅰ ranged from79 .3% to 99 .5% % ,only several sequences were identical .The results showed that α1 andα2 regions of chicken M HCⅠ shared higher amino acid homology with duck ,between 57 .6%‐64 .1% ,and followed by human and mouse .In the phylogenetic tree all amino acid sequences of α1 andα2 regions from chickens belonged to two clusters together ,no correlation with the chicken breeds .Chicken had a closer genetic re‐lationship with duck ,and genetic distance of other species was farther .The results show that chicken M HCⅠ molecules showed high polymorphism in the evolutionary process ,but the polymorphism located mainly in peptide‐binding region of α1 andα2 under pressure of the pathogen .关键词
M HCⅠ类分子/序列分析/淮南麻黄鸡Key words
Huainan partridge chicken/M HC class Ⅰ/sequence analysis分类
农业科技引用本文复制引用
戴银,王承志,沈学怀,赵瑞宏,胡晓苗,侯宏艳,潘孝成,周学利,张丹俊..淮南麻黄鸡 MHC Ⅰα链基因的克隆及序列分析[J].动物医学进展,2015,(2):29-33,5.基金项目
国家自然科学基金项目（31302044）；国家蛋鸡产业技术体系项目（C A RS-41）；安徽省现代农业肉禽产业发展资金；院科技创新团队资助项目 （）
