动物医学进展Issue(2)：39-42,4.
鸭疫里默杆菌 mazp 基因克隆表达及免疫原性分析
Cloning Expression and Immunogenicity of mazp from Riemerella Anatipesti f er
王秀祯 1罗永芳 1夏文君 1罗雪刚 1彭少静 1周念 1李继祥1
作者信息
- 1. 西南大学荣昌校区动物医学系，重庆402460
- 折叠
摘要
Abstract
To study the mutual protective antigen of Riemerella anatipesti f er (RA ) strains ,the reserch cloned and expressed membrane‐associated zinc metalloprotease (mazp) gene of RA ,and studied its ex‐pression immunogenicity using animal test .The specific primers were designed based on the nucleotide se‐quence of mazp gene of RA‐GD strain and the mazp gene of RA‐AF strain was cloned and sequenced .Com‐pared with other strains through BLAST analysis ,the 1 326 bp nucleotide sequence of mazp gene of RA -AF strain was highly conserved .Then the mazp gene fragment was cloned into pET‐32a (+ ) to construct expression plasmid pET‐32a‐mazp .It expressed recombination protein in E .coli BL21 (DE3) when in‐duced by isopropyl thiogalactoside (IPTG) .Subcutaneous inoculation of the recombination protein in 5‐day‐old ducklings gave adequate protective index (PI=62 .4) against challenge with RA‐AF strain (sero‐type 2) 15 days after vaccination ,while the protection (PI=77 .8) was obtained challenged with RA‐BSY strain (serotype 1) .The antiserum of recombination protein was used as antibody to immunize ducklings , delaying morbility and death was obtained .The result indicated the recombination protein mazp has immu‐nogenicity ,and may play a role in protecting duck from infection of Riemerella anatipesti f er (sreotype 1 , 2) .关键词
鸭疫里默杆菌/mazp基因/免疫原性/保护指数Key words
Riemerella anatipesti f er/mazp gene/immunogenicity/protective index分类
生物科学引用本文复制引用
王秀祯,罗永芳,夏文君,罗雪刚,彭少静,周念,李继祥..鸭疫里默杆菌 mazp 基因克隆表达及免疫原性分析[J].动物医学进展,2015,(2):39-42,4.
