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一株扭角羚弗格森埃希菌的分离鉴定及药敏试验

叶泥 权自芳 王彬 谌利民 杨艳 颜其贵

动物医学进展Issue(2):69-72,4.
动物医学进展Issue(2):69-72,4.

一株扭角羚弗格森埃希菌的分离鉴定及药敏试验

Identification of Escherichia Fergusonii and Analysis of Its Drug Resistance

叶泥 1权自芳 1王彬 1谌利民 2杨艳 2颜其贵1

作者信息

  • 1. 四川农业大学动物医学院,四川雅安625014
  • 2. 四川省广元市青川县青溪镇唐家河管理处,四川广元628019
  • 折叠

摘要

Abstract

A strain of bacillus was found in the liver of a dead youth yakin which was sent by nature reserve in Sichuan province in May 2014 .We isolated and identified this bacillus ,and furthermore ,biochemical test and drug sensitive test were analyzed .16 S rDNA sequence of this bacillus was amplified by polymer‐ase chain reaction (PCR) ,and by sequencing the PCR products ,we got the sequences of 16 S rDNA .Ac‐cording to the Blast results and biochemical characteristics ,the biological classification of this bacillus was obtained ,and then ,through the drug sensitive test ,the degree of bacterial drug resistance was revealed . Experimental results show that the homology was found to be more than 99% after blasting the 16 S rDNA sequences in NCBI database and combining with the biochemical results ,the strain was identified as Esche‐richia f ergusonii .The drug sensitive test results show that the strain was highly resistant to ampicillin , and sensitive to ciprofloxacin ,florfenicol ,ceftazidime .

关键词

弗格森埃希菌/生化试验/药敏试验

Key words

Escherichia f ergusonii/biochemical test/drug sensitive test

分类

农业科技

引用本文复制引用

叶泥,权自芳,王彬,谌利民,杨艳,颜其贵..一株扭角羚弗格森埃希菌的分离鉴定及药敏试验[J].动物医学进展,2015,(2):69-72,4.

基金项目

教育部长江学者和创新团队发展计划项目 ()

动物医学进展

OA北大核心CSTPCD

1007-5038

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