动物医学进展Issue(3)：1-4,5,5.
金黄色葡萄球菌表面蛋白SdrD的原核表达及抗体制备
Prokaryotic Expression and Antibody Preparation of SdrD Surface Protein of Staphylococcus aureus
摘要
Abstract
In this study,specific primers were designed and synthesized based on the SdrD gene of Staphy-lococcus aureus and used to amplify the gene fragment using the genomic DNA of S.aureus standard strain as template.A gene fragment of 2 043 bp in length was obtained.The ORF was ligated into pET26b ex-pression vector and transformed into E.coli BL2 1 (DE3 )for recombinant expression under induction of IPTG.The results of SDS-PAGE indicated that the recombinant protein with molecular weight of 9 9 ku was successfully expressed.The target protein was purified using immunoaffinity chromatography and used to immunize New Zealand white rabbits to prepare polyclonal antibodies.ELISA demonstrated that the titers of antiserum reached 1∶23 000.This study lays a foundation for developing engineered vaccines and immune detection methods for S.aureus.关键词
金黄色葡萄球菌/表面蛋白/SdrD基因/重组表达/抗体制备Key words
Staphylococcus aureus/surface protein/SdrD gene/recombinant expression/antibody preparation分类
农业科技引用本文复制引用
杜欣军,王学连,李萍,耿洁洁,张洪娟,王硕..金黄色葡萄球菌表面蛋白SdrD的原核表达及抗体制备[J].动物医学进展,2015,(3):1-4,5,5.基金项目
“十二五”农村领域国家科技计划项目（2012BAD29B02）；教育部科学技术研究重点项目 （）
