动物医学进展Issue(3)：9-12,4.
布鲁菌外膜蛋白Omp22基因的原核表达与生物信息学分析
Cloning,Prokaryotic Expression and Bioinformatics Analysis of Outer Membrane Protein Omp2 2 Gene of Brucella
摘要
Abstract
The Omp22 gene was cloned and analyzed with bioinformaties after the prokaryotic expression. According to the Brucella melitensis vaccine strain M5-90 gene in GenBank,a target gene fragment with 639 bp was amplified by PCR.The cloning recombinant plasmid pMD-20T-Omp22 was constructed and transformed into the E.coli DH5α.The prokaryotic expression recombinant plasmid pET-28a-Omp22 was constructed after sequencing,and transformed into the E.coli BL2 1 (DE3 ).The fusion protein was ex-pressed under induction by IPTG,and identified with Western blot.The result showed that the Omp22 gene was cloned successfully and the prokaryotic expression vector pET-28a-Omp22 was successfully es-tablished and expressed in E.coli BL21(DE3).DNA Man and BioeditT softwares were used to analyze the secondary structure of the Omp22 fusion protein,the alpha helix accounts for 22.17%,the extended strand 19.81%,the beta turn 2.83%,and the random coil 55.19%.The protein has higher hydrophilicity.关键词
布鲁菌/外膜蛋白22 基因/克隆/原核表达/生物信息学分析Key words
Brucella/Omp22 gene/cloning/prokaryotic expression/bioinformatics analysis分类
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朱华培,成鹰,焦寒伟,杜丽,王凤阳,赵天靖,徐开莲,贾晓晓,郭莳雨,史巧芸,庞峰,荣辉,张珈宁..布鲁菌外膜蛋白Omp22基因的原核表达与生物信息学分析[J].动物医学进展,2015,(3):9-12,4.基金项目
“863”计划项目 （）
