动物医学进展Issue(3)：13-16,4.
三黄鸡临床血管瘤病例中分离出A亚群与J亚群禽白血病病毒
Isolation of Subgroup A and J ALV in the Clinical Hemangioma of Three-Yellow Chicken
摘要
Abstract
In order to understand the avian leukosis virus epidemic situation in Guangxi chickens,two strains of avian leukosis virus subgroup A and J were isolated in Three-yellow chickens in the cultured DF-1 cells,which were identified by ALV p2 7 detection and PCR amplification and sequence analysis of avian leukosis virus gp85 gene,and then were named as HG01-A and HG01-J,respectively.The results of varia-tion analysis of gp85 gene indicated that the homology between HG01-A and 7 reference strains was 85.8%-87.5%,and shared the highest homology with MQNCSU at 87.5%,shared the homology with RSA at 86.9%.The results of variation analysis of gp85 gene indicated that the homology between HG01-J and 7 reference strains was 84.0%-93.8%,and shared the highest homology withXX2-08 and SCSM01 at 92.8%,shared the homology withHPRS1-3 at 90.2%.The present results indicated that it the first report that there was the co-infection of ALV-A and ALV-J in the Guangxi Three-yellow chickens,and provided new epidemiological information of avian leucosis virus in local chicken.关键词
共感染/A亚群禽白血病病毒/J亚群禽白血病病毒/gp85/聚合酶链反应Key words
co-infection/ALV-J/ALV-A/gp85/PCR分类
农业科技引用本文复制引用
王培坤,毕玉彧,秦丽莉,邹广珍,彭昊,韦平..三黄鸡临床血管瘤病例中分离出A亚群与J亚群禽白血病病毒[J].动物医学进展,2015,(3):13-16,4.基金项目
国家公益性行业（农业）科研专项（201203055）；国家现代农业产业技术体系广西肉鸡产业创新团队建设项目 （）
