动物医学进展Issue(3)：28-31,4.
牛皮蝇幼虫蛋白表达谱及一种高表达蛋白的分离纯化
Protein Expression Profiling of Hypoderma Larvae and Purification of A Protein with High Expression Level
摘要
Abstract
The aim of this study was to reveal the protein expression profiling of the Hypoderma larva in-fecting yaks,in order to provide data for the basic research of Hypoderma and the prevention of hypoder-mosis.Total protein of the Hypodermasinense larva collected from subcutaneous tissues of yak was extrac-ted and analyzed by SDS-PAGE.A protein with high was purified using gel exlusion and ion-exchange chromatography and two samples of Hypoderma larvae whose total protein profiling displayed great differ-ence were separated and identified by two-dimensional electrophoresis and mass spectrometry.The results showed that the protein expression profile of larvae varied among individuals,and approximately 23% of the individuals contained a protein with molecular weight of about 78 ku,which was purified by above methods.A total of 312±24 and 284±13 protein spots were obtained respectively by two-dimensional e-lectrophoresis and four of these proteins were analyzed by mass spectrometry,however,none of the pro-teins were identified with high score matches.This study obtained the protein expression profiling of Hy-poderma larvae and purified a protein,laying a foundation for further studies on the gene expression and protein functions of Hypoderma larvae.关键词
牦牛/牛皮蝇幼虫/双向电泳/蛋白质纯化Key words
yak/Hypoderma larva/two-dimensional electrophoresis/protein purification分类
农业科技引用本文复制引用
付伟,李鹏飞,娄亚南,金素钰,黄林,林亚秋,郑玉才..牛皮蝇幼虫蛋白表达谱及一种高表达蛋白的分离纯化[J].动物医学进展,2015,(3):28-31,4.基金项目
四川省应用基础研究资助项目 （）
