动物医学进展Issue(3)：32-34,3.
牛副流感病毒3型N基因的克隆与系统进化分析
Cloning and Phylogenetic Analysis of N Gene of Bovine Parainfluenza Virus Type 3
摘要
Abstract
The N gene of bovine parainfluenza virus type 3 from Daqing was amplified and its homology was analyzed.A pair of primers were designed and the N gene of BPIV-3 was amplified by PCR.The gene was linked to the restriction enzyme cutting sites of BamH I and Hind Ⅲ of cloning vector,the nucleotide se-quence of the inserted fragment was determined and analyzed by molecular biological software.The PCR a-nalysis results showed that the BPIV-3-N was 1 548 bp,and encoded 515 amino acids.It indicated that the BPIV-3-N gene shared high homology with BPIV-3-N of 910N isolated from Japan in Genbank,the homol-ogy of nucleotides was 99.7%,the homology of amino acids was 100%.关键词
牛副流感病毒3型/N基因/序列分析Key words
Bovine parainfluenza virus type 3/N gene/sequence analysis分类
农业科技引用本文复制引用
薛娟,曹思,张峣,冉旭华,闻晓波..牛副流感病毒3型N基因的克隆与系统进化分析[J].动物医学进展,2015,(3):32-34,3.基金项目
“十二五”农村领域国家科技计划课题（2012BAD12B03-3）；黑龙江八一农垦大学“大学生创新创业训练计划”；国家自然科学基金项目 （）
