动物医学进展Issue(3)：59-63,5.
猪伪狂犬病病毒NP株的分离鉴定
Isolation and Identification of Porcine Pseudorabies Virus NP Strain
摘要
Abstract
In mid 2013,one strain of pseudorabies virus (PRV)was isolated from organs of dead piglets with symptoms of PRV-like infection,which were collected from a large-scale farms in Fuj ian Province,it was named PRV NP strain.The symptoms of PRV infection were characterized by nervous system disor-ders and deaths in newborn piglets as well as reproductive failure in sows.In addition,the PCR and se-quencing results were consistent with expected results;PRV was able to propagate in MDCK cells and cause a typical cytopathogenic effect.The tissue culture infective dose (TCID50)was 10-9.13/0.1 mL con-firmed by the Reed-Muench methods.The typical pseudorabies clinical symptoms were presented in rabbits and mice inoculated with the isolated virus.These results demonstrated that a new wild pseudorabies virus has been isolated,which lay a foundation for the further research on the molecular epidemiology of pseud-orabies virus.关键词
猪伪狂犬病病毒/分离/鉴定Key words
pseudorabies virus/isolation/identification分类
农业科技引用本文复制引用
林群群,郑小香,陈秋勇,陈鹏强,曾显成,胡崇伟,修金生..猪伪狂犬病病毒NP株的分离鉴定[J].动物医学进展,2015,(3):59-63,5.基金项目
福建省农业科学技术项目（2014-01）；福建省自然科学基金项目 （）
