动物营养学报Issue(4)：1019-1027,9.DOI:10.3969/j.issn.1006-267x.2014.04.022
复合菌固态发酵培养基及发酵工艺的优化
Optimization of Compound Bacteria Solid-State Fermentation Medium and Fermentation Process
摘要
Abstract
This experiment was conducted to study the optimization of compound bacteria solid-state fermenta-tion medium and fermentation process. The mixing experimental design and uniform experimental design by DPS software were used, combined into 14 kinds of solid-state fermentation medium and 14 kinds of fermenta-tion process. Determining the contents of viable count, β-glucan, mannan and organic acid to filter the fine components and optimized the fermentation process. The results showed as follows: 1 ) the optimized com-pound bacteria solid-state fermentation medium was wheat bran 52 . 69%, corn bran 20 . 45%, corn flour 16 . 07%, rice bran 4 . 09%, cottonseed meal 1 . 86%, soybean meal 3 . 99% and inorganic salt 0 . 85%; 2 ) the optimized fermentation process was moisture 60%, pH 8. 0, temperature 30 ℃ and time 46 h. The indexes of optimal combination were viable count 6. 71 í108 CFU/g, glucan content 279. 83 mg/g, mannan content 156. 82 mg/g, tartaric acid content 2. 78 mg/g, malic acid content 8. 28 mg/g, citric acid content 1. 34 mg/g and butanedioic acid content 2. 54 mg/g. In conclusion, this experiment optimizes the compound bacteria sol-id-state fermentation medium and fermentation process.关键词
复合菌/活性物质/发酵培养基/发酵工艺Key words
compound bacteria/active material/fermentation medium/fermentation process分类
农业科技引用本文复制引用
刘国娟,刘大程,卢春芳,胡红莲,高民..复合菌固态发酵培养基及发酵工艺的优化[J].动物营养学报,2014,(4):1019-1027,9.基金项目
国家自然科学基金(31260560) （31260560）
现代农业(奶牛)产业技术体系建设专项资金资助(CARS-37) （奶牛）
