动物营养学报Issue(4)：1120-1126,7.DOI:10.3969/j.issn.1006-267x.2014.04.034
金雀异黄素对多囊卵巢综合征大鼠卵巢组织中Bcl-2及 Bax mRNA表达的影响
Effects of Genistein on Expressions of Bcl-2 and Bax mRNA in Ovarian Tissue of Polycystic Ovary Syndrome Rats
摘要
Abstract
This experiment was conducted to study the effects of genistein ( GEN) on the expressions of anti-apoptotic gene Bcl-2 mRNA and pro-apoptotic gene Bax mRNA in ovarian tissue of polycystic ovary syndrome ( PCOS) rats. Ninety rats were divided into six groups: control group, model group, GEN low dose group, GEN middle dose group, GEN high dose group and estrogen group, respectively. The insulin combined with human chorionic gonadotropin molding method was used to establish PCOS animal models. After modeling, ten rats were selected from each group to carry out the test. The rats in dose groups were given 5 , 10 and 20 mg/kg GEN, respectively, and the rats in estrogen group were given 0. 5 mg/kg diethylstilbestrol. All compounds were administered intragastric administration on 15 consecutive days and observed a estrous cycle of 10 days. The expressions of Bcl-2 and Bax mRNA in rat ovarian tissue were examined by in situ hybridization. The activities of superoxide dismutase, catalase and glutathione peroxidase, and malondialdehyde content in rat ovarian tissue were determined. The results showed as follows: compared with PCOS model group, GEN could raise the expression of Bcl-2 mRNA and weaken the expression of Bax mRNA. And GEN could regulate the activities of antioxidant enzymes in rat ovarian tissue, which was as the same as the estrogen. In conclu-sion, GEN can enhance the expression of Bcl-2 mRNA and reduce the expression of Bax mRNA in rat ovarian tissue. It is presumed that it is one of the mechanisms of GEN improving ovarian function of PCOS.关键词
金雀异黄素/多囊卵巢综合征/卵巢/Bcl-2 mRNA/Bax mRNAKey words
genistein/polycystic ovary syndrome/ovary/Bcl-2 mRNA/Bax mRNA分类
农业科技引用本文复制引用
迟晓星,张明玉,张涛,郑丽娜,张丽媛..金雀异黄素对多囊卵巢综合征大鼠卵巢组织中Bcl-2及 Bax mRNA表达的影响[J].动物营养学报,2014,(4):1120-1126,7.基金项目
黑龙江省高校青年学术骨干项目(1252G040) （1252G040）
哈尔滨医科大学校内基金(dq201104) （dq201104）
