动物营养学报Issue(6)：1486-1495,10.DOI:10.3969/j.issn.1006-267x.2014.06.008
植物蛋白质复合物替代凡纳滨对虾饲料中鱼粉的研究
RePlacement of Fish Meal with Plant Protein Mixture in Diets for Pacific White ShrimP(Litopenaeus vannamei)
摘要
Abstract
This experiment was conducted to study the feasibility of replacement of fish meal with plant protein mixture(extruded soybean meal ∶ peanut meal ∶ corn gluten meal = 38% ∶17% ∶45%)in diets for Pacific white shrimp(Litopenaeus vannamei),and to find out the optimal replacement proportion. A total of 720 Pacific white shrimps with an average body weight of(0.52±0.01)g were randomly distributed into 6 groups with 3 replicates per group and 40 shrimps per replicate. Six isonitrogenous and isoenergetic diets were formulated with 3.91%,7.82%,11.73%,15.64% and 23.46% plant protein mixture to replace 10%,20%,30%,40% and 60% fish meal,respectively. The experiment lasted for 56 d. The results showed as follows:different replace-ment proportions of fish meal with plant protein mixture showed no significant effects on survival rate( SR), hepatopancreas-somatic index and the contents of crude lipid and ash in whole body(P>0.05). When the re-placement proportion was over 40%,weight gain rate(WGR),specific growth rate(SGR),protein efficien-cy ratio(PER)and the contents of total protein and glucose in serum were significantly decreased(P<0.05), while feed conversion ratio( FCR)was significantly increased( P<0.05). When the replacement proportion was over 30%,protein retention ratio( PRR),whole body crude protein content and serum total cholesterol content were significantly decreased( P<0.05),while serum triglyceride content was significantly increased ( P<0.05). Replacement of 30% fish meal with plant protein mixture had no significant effects on the activities of acid phosphatase( ACP),alkaline phosphatase( AKP),surperoxide dismutase( SOD)and nitric oxide synthetase(NOS)in serum(P>0.05),while significantly decreased the apparent digestibility of protein,lip-id,energy and most of essential amino acids including methionine,phenylalanine,valine,histidine,arginine and threonine(P<0.05). Comprehensive analysis of growth,feed utilization and nutrient apparent digestibili-ty,the optimal proportion of plant protein mixture(extruded soybean meal ∶ peanut meal ∶ corn gluten meal =38% ∶17% ∶45%)to replace fish meal is 20% in the basal diet for Pacific white shrimp,which containing 40%protein and 30% fish meal.关键词
凡纳滨对虾/鱼粉/植物蛋白质复合物/替代/养分表观消化率Key words
Pacific white shrimp(Litopenaeus vannamei)/fish meal/plant protein mixture/replacement/apparent nutrient digestibility分类
农业科技引用本文复制引用
杨奇慧,谭北平,周小秋,董晓慧,迟淑艳,刘泓宇..植物蛋白质复合物替代凡纳滨对虾饲料中鱼粉的研究[J].动物营养学报,2014,(6):1486-1495,10.基金项目
农业部公益性行业（农业）科研专项（201003020）；广东高校优秀青年创新人才培育计划资助项目（1009324）；广东自然科学基金项目（S2012040007863）；广东海洋大学自然科学基金项目 （）
