动物营养学报Issue(6)：1503-1512,10.DOI:10.3969/j.issn.1006-267x.2014.06.010
饲料中添加枯草芽孢杆菌对吉富罗非鱼幼鱼生长性能、免疫力和抗氧化功能的影响
Effects of Dietary Bacillus subtilis on Growth Performance,Immunity and Anti-oxidation Function of Juvenile Genetic ImProvement of Farmed TilaPia(GIFT,Oreochromis niloticus)
摘要
Abstract
The objective of this study was to evaluate the effects of dietary Bacillus subtilis on growth perform-ance,immunity and anti-oxidation function of juvenile genetic improvement of farmed tilapia( GIFT,Oreo-chromis niloticus),and to find out the appropriate supplemental level of Bacillus subtilis in diets. Juvenile GIFT with an initial body weight of(0.43±0.02)g were fed 7 experimental diets supplemented with 0(con-trol group),0.03%,0.06%,0.09%,0.12%,0.15% and 0.18%( the corresponding actual living bacteria number was 0.20×102 ,0.69×1010 ,1.38×1010 ,2.05×1010 ,2.82×1010 ,3.55×1010 and 4.23×1010 cfu / kg,re-spectively)Bacillus subtilis for 56 days,respectively. Each group had 3 replicates with 50 fish per replicate. The results showed as follows:the supplementation of 0. 03% to 0. 18% Bacillus subtilis had no significant effect on growth performance(P>0.05). After supplementing different levels of Bacillus subtilis in diets,the activities of aspartate aminotransferase(AST)and alanine aminotransferase(ALT)and the content of maleic dialdehyde(MDA)in serum were declined at different degrees,while the spleen index and the content of complement 3(C3),the activities of lysozyme( LSZ),alkaline phosphatase( ALP),total superoxide dis-mutase(T-SOD)and catalase(CAT),and total anti-oxidation capacity(T-AOC)in serum were enhanced at different degrees. When the Bacillus subtilis supplemental levels were 0.12% to 0.18%,the effects were signif-icant(P<0.05). Cumulative survival rate in groups with the Bacillus subtilis supplemental levels of 0.09%, 0.12%,0.15% and 0.18% was significantly improved after being challenged with Aeromonas hydrophila in 168 h(P<0.05),and the time of death was delayed. It is concluded that the supplementation of 0.12% to 0.18% Bacillus subtilis can improve the immunity and anti-oxidation function of juvenile GIFT. Considering the cost of adding Bacillus subtilis,the 0.12%(2.82×1010 cfu / kg)Bacillus subtilis is optimum.关键词
枯草芽孢杆菌/吉富罗非鱼/生长性能/免疫力/抗氧化功能Key words
Bacillus subtilis/GIFT/growth performance/immunity/anti-oxidation function分类
农业科技引用本文复制引用
程远,黄凯,黄秀芸,钟灵香,武林华,黄清,唐丽宁..饲料中添加枯草芽孢杆菌对吉富罗非鱼幼鱼生长性能、免疫力和抗氧化功能的影响[J].动物营养学报,2014,(6):1503-1512,10.基金项目
国家自然科学基金（31260640）；“十二五”国家科技支撑计划（2012BAD25B04）；广西“十二五”重大科技专项（1123010）；广西科技攻关项目（0992014-2）；广西南宁市科技攻关项目 （）
