动物营养学报Issue(6)：1527-1534,8.DOI:10.3969/j.issn.1006-267x.2014.06.013
乙酸钠和β-羟丁酸钠对奶牛乳腺上皮细胞乳脂和乳蛋白合成相关基因表达的影响
Effects of Sodium Acetate and Sodium β-Hydroxybutyrate on ExPressions of Genes Involved in Milk Fat and Protein Synthesis in Bovine Mammary EPithelial Cells
摘要
Abstract
The aim of this study was to determine the effects of sodium acetate and sodium β-hydroxybutyrate on expressions of genes involved in milk fat and protein synthesis in bovine mammary epithelial cells (BMECs). The study was consisted of two parts,and completely random single-factor designs were adopted. Part 1:single supplementation test,the supplemental concentrations of sodium acetate and sodium β-hydroxy-butyrate were 0( control),6. 00,9. 00,12. 00 and 15. 00 mmol/ L,and 0( control),0. 80,1. 60 and 2.40 mmol/ L,respectively. Part 2:mixed supplementation test,based on data from part 1,the total supple-mental concentration was the sum of optimal supplemental concentrations of sodium acetate and sodium β-hydroxybutyrate;three ratios of sodium acetate to sodium β-hydroxybutyrate(1 ∶ 1,2 ∶ 1 and 4 ∶ 1)were de-signed,and the treatment of no supplementation was used as a control group. The results showed as follows:1)compared with control group,12.00 mmol/ L sodium acetate significantly increased the expression levels of acetyl-CoA carboxylase(ACC),fatty acid synthetase(FAS),diacylgycerol acyltransferase(DGAT),acetyl-CoA synthetase 2(ACSS2),peroxisome prolifertor receptor(PPARG),κ-casein(CSN3)genes and the con-tent of triglyceride(TAG)in BMECs(P<0.05). 2)Compared with control group,2.40 mmol/ L sodium β-hydroxybutyrate significantly increased the expression levels of ACC,FAS,ACSS2,PPARG and mammalian target of rapamycin(mTOR)genes and the content of TAG in BMECs(P<0.05). 3)When the ratios of sodi-um acetate to sodium β-hydroxybutyrate were 2∶1 and 4 ∶1,The supplementation of sodium acetate and sodiumβ-hydroxybutyrate at different mixed ratios significantly up-regulated the expression levels of genes involved in milk fat synthesis at different extents. The content of TAG in BMECs were significantly increased compared with when the ratio was 1 ∶ 1 and control group( P < 0. 05). In conclusion,the mixed supplementation of 9.60 mmol/ L sodium acetate and 4.80 mmol/ L sodium β-hydroxybutyrate are optimal,considering its simula-tive effects on milk fat and protein synthesis.关键词
奶牛乳腺上皮细胞/乙酸钠/β-羟丁酸钠/乳脂/乳蛋白Key words
bovine mammary epithelial cells/sodium acetate/sodium β-hydroxybutyrate/milk fat/milk pro-tein分类
农业科技引用本文复制引用
塔娜,李红磊,侯先志,考桂兰,高民,李大彪..乙酸钠和β-羟丁酸钠对奶牛乳腺上皮细胞乳脂和乳蛋白合成相关基因表达的影响[J].动物营养学报,2014,(6):1527-1534,8.基金项目
国家自然科学基金“应用三维细胞培养模型研究脂肪酸和激素对奶牛乳腺上皮细胞乳脂肪合成的影响及分子机理”（31360559）；现代农业（奶牛）产业技术体系建设专项资金 （）
