动物营养学报Issue(6)：1601-1607,7.DOI:10.3969/j.issn.1006-267x.2014.06.022
嗜酸乳杆菌活菌体和热灭活菌体对 IPEC-J2细胞的黏附和黏附拮抗效应
Adhesion and Adhesion Antagonistic Effect of Live and Heat-Killed Lactobacillus acidophilus on IPEC-J2 Cells
俞涵丽 1方华 1蔡旋 2徐建雄2
作者信息
- 1. 上海源耀生物股份有限公司，上海 201316
- 2. 上海交通大学农业与生物学院，上海 200240
- 折叠
摘要
Abstract
The adhesion and adhesion antagonistic effect of live and heat-killed Lactobacillus acidophilus on IPEC-J2 cells were studied in vitro. IPEC-J2 cells were treated with live and heat-killed Lactobacillus acidophi-lus,and then stained the cells and observed the adhesive property. Moreover,the inhibition ratio of live and heat-killed Lactobacillus acidophilus to inhibit the adhesion of Escherichia coli on IPEC-J2 cells through three treatments(competitive inhibition,exclusion inhibition and displacement inhibition)was assessed by eosin-methylene blue agar(EMB)culture medium. The results showed as follows:live and heat-killed Lactobacillus acidophilus all could adhere well to the IPEC-J2 cells. The number of heat-killed bacteria adhering to the per cell[(40±2)CFU]was higher than the number of live bacteria adhering to the per cell[(20±2)CFU]. The two forms of Lactobacillus acidophilus had a good ability to inhibit the Escherichia coli adhere to IPEC-J2 cells. In competitive and displacement inhibition experiments,live bacteria showed a significantly better inhibi-tion ratio than heat-killed bacteria(P<0.05),whereas,in exclusion inhibition experiment,heat-killed bacteria showed a significantly better inhibition ratio than live bacteria( P<0.05). In conclusion,live and heat-killed Lactobacillus acidophilus can adhere well to the IPEC-J2 cells,and are able to reduce the adhesion and inva-sion to IPEC-J2 cells by Escherichia coli. Heat-killed Lactobacillus acidophilus inhibits Escherichia coli adhe-sion mainly through competition for host cell-binding receptors. Apart from competition for host cell-binding re-ceptors,some antibacterial substances from live Lactobacillus acidophilus are able to inhibit the growth and re-production of Escherichia coli.关键词
嗜酸乳杆菌/活菌体/热灭活菌体/大肠杆菌/黏附/抑制黏附/抑菌能力Key words
Lactobacillus acidophilus/live bacteria/heat-killed bacteria/Escherichia coli/adhesion/inhibi-tion adhesion/antimicrobial ability分类
农业科技引用本文复制引用
俞涵丽,方华,蔡旋,徐建雄..嗜酸乳杆菌活菌体和热灭活菌体对 IPEC-J2细胞的黏附和黏附拮抗效应[J].动物营养学报,2014,(6):1601-1607,7.
