动物营养学报Issue(6)：1624-1631,8.DOI:10.3969/j.issn.1006-267x.2014.06.025
肝脏营养物质代谢的仔猪肝-门静脉血插管技术的建立
Establishment of Techniques for ImPlanting HePatic-Portal Catheters in Piglets for Studying HePato-Nutrients Metabolism
摘要
Abstract
This study was aimed to establish techniques for implanting arterial and venous catheters in piglets to provide technical support for studying hepato-nutrients metabolism. A total of 18 healthy Duroc ×Landrace × Yarkshire castrated piglets weighted(20.0±1.1)kg were selected,and hepatic vein,portal vein,mesenteric vein,carotid artery catheters were implanted respectively. After 5 days recovery period,a 1% solution of p-a-mino hippurate(pAH)was infused continuously into the mesenteric catheters. The blood samples from hepatic vein,portal vein and carotid artery were collected at 0.5 h before feeding and at 0.5,1.5,3.0,5.0 and 7.5 h after feeding,respectively. Hepatic vein and portal vein blood flow were detected. The results showed that the average time of keeping unobstructed in blood vessels of the implanted catheter system was 16 days. Hepatic vein and portal vein blood flow velocity increased after feeding. Portal vein flowe reached 2.80 L /(kg·h)and 981.40 mL / min. Hepatic vein flow reached 4.04 L /(kg·h)and 1 414.65 mL / min. Twelve of the eighteen test piglets can be successfully used for studying hepato-nutrients metabolism. Hepatic vein and portal vein blood flow tends to similarly change.关键词
仔猪/肝脏代谢/门静脉/肝静脉/插管技术Key words
piglet/liver metabolism/portal vein/hepatic vein/catheter techniques分类
农业科技引用本文复制引用
郑培培,包正喜,李鲁鲁,胡长敏,赵胜军,刘东明,黄飞若..肝脏营养物质代谢的仔猪肝-门静脉血插管技术的建立[J].动物营养学报,2014,(6):1624-1631,8.基金项目
国家重点基础研究计划（973计划） （）
