动物营养学报Issue(8)：2397-2407,11.DOI:10.3969/j.issn.1006-267x.2014.08.047
饲料中添加低聚木糖对凡纳滨对虾幼虾生长性能、非特异性免疫力、抗氧化功能及抗对虾白斑综合征病毒能力的影响
Xylo-Oligosaccharides Supplementation Affects Growth Performance, Hon-Specific Immunity, Antioxidant Function and Anti-White Spot Syndrome Virus Capacity of Juvenile Litopenaeus vannamei
摘要
Abstract
This experiment was aimed to study whether xylo-oligosaccharides( XOS)supplementation could affect non-specific immunity,antioxidant function and anti-white spot syndrome virus( WSSV)capacity of ju-venile Litopenaeus vannamei. Eight hundred juvenile Litopenaeus vannamei with an initial body weight of (0.67±0.02)g were randomly assigned to 4 groups with 4 replicates per group and 50 shrimps per replicate. Shrimps in the 4 groups were fed diets supplemented with 0( G0 group,as control group ),200( G200 group),400( G400 group ) and 600 mg/kg( G600 group ) XOS for 6 weeks,respectively. After the 6 weeks’ feeding trial, shrimps were injected with WSSV. The results showed that the weight gain rate ( WGR),special gain rate( SGR),protein efficiency ratio( PER)and protein deposition rate( PDR)were significantly increased and the feed conversion ratio ( FCR ) was significantly decreased when adding 200 mg/kg in the diet( P<0.05). Dietary XOS could increase the total haemocyte count( THC),and that in G400 group was significantly higher than that in control group and G600 group( P<0.05). The activities of phenoloxidase( PO)and lysozyme( LZM)in serum and LZM in gill were significantly increased by adding XOS in the diet( P<0.05). Compared with control group,the activities of superoxide dismutase( SOD)and peroxidase( POD)in serum and hepatopancreas and serum total antioxidant capacity( T-AOC)in G200 group were significantly increased(P<0.05),the hepatopancreas T-AOC in experimental groups was significantly increased(P<0.05),and the activity of hepatopancreas anti-superoxide anion radical(Anti-O-2·)in G400 group was significantly increased(P<0.05),while the hepatopancreas malondialdehyde(MDA)content in G400 group and the serum MDA content in experimental groups were significantly reduced( P<0.05). After 72 h of WSSV injection,the cumulated mortality rate( CMR)in experimental groups was significantly de-creased compared with control group( P<0.05),and the relative percentage survival( RPS)in G200,G400 and G600 groups was 15.2%,18.2% and 15.2%,respectively. In conclusion,suitable amount of XOS in the diet can improve growth performance,non-specific immunity,antioxidant function and anti-WSSV capacity of juvenile Litopenaeus vannamei.关键词
低聚木糖/凡纳滨对虾/生长性能/非特异性免疫指标/抗氧化指标/抗病力Key words
xylo-oligosaccharides/Litopenaeus vannamei/growth performance/non-specific immune indi-ces/antioxidant indices/disease resistance分类
农业科技引用本文复制引用
陈晓瑛,曹俊明,黄燕华,王国霞,莫文艳,陈冰,赵红霞,付晶晶..饲料中添加低聚木糖对凡纳滨对虾幼虾生长性能、非特异性免疫力、抗氧化功能及抗对虾白斑综合征病毒能力的影响[J].动物营养学报,2014,(8):2397-2407,11.基金项目
国家星火计划项目（2012GA780002）；广东省自然科学基金团队对虾项目（10351064001000000）；广州市科技计划项目（2011J4300089）；广州市应用基础研究专项 （）
