动物营养学报Issue(11)：3298-3306,9.DOI:10.3969/j.issn.1006-267x.2014.11.016
不同蛋白质饲料对宣汉黄牛瘤胃固相黏附纤维降解微生物数量的影响
Different Protein Feeds Affect Populations of Cellulosis Microbes Adherent to Solid Fractions in Rumen of Xuanhan Yellow Cattle
摘要
Abstract
The objective of this paper was to investigate the effects of three protein feeds ( soybean meal, rape-seed meal and cottonseed meal) on the populations of cellulosis microbes adherent to solid fractions in rumen of Xuanhan yellow cattle. Four healthy rumen-cannulated Xuanhan yellow cattle with similar body weight were used in a 4×4 Latin Square design. There were 4 periods with 10 days in each period. Cattle in basal group were fed a basal diet, and those in experimental groups were fed experimental diets, in which concentrate in basal diet were replaced by soybean meal ( SBM group ) , rapeseed meal ( RSM group ) and cottonseed meal ( CSM group) at 15% of basal diet, respectively. The fluorescent quantitation PCR technique was introduced to analyze the change of the populations of fungi, protozoa, R. albus, R. flavefaciens, and F. succinogenes ad-herent to solid fractions in rumen after 1, 3, 5, 7 and 9 hours of feeding. The results showed as follows: 1) with the increasing of time after feeding, there was no the same trend in different groups on the population of cellulosis microbes adherent to solid fractions in rumen. The populations of cellulosis microbes in SBM group reached the highest concentrated at 5 and 7 hours after feeding, and those in CSM group almost did at 7 hours after feeding. 2) Compared among means at different time after feeding of different groups, the population of fungi in CSM group was significantly higher than that in RSM and SBM groups ( P<0.05) , and the population of protozoa, R. flavefaciens and F. succinogenes in RSM group were significantly higher than those in SBM and CSM groups ( P<0.05 or P<0.01) . In conclusion, different protein feeds significantly affect the cellulosis microbes adherent to solid fractions in rumen, and rapeseed meal has better promoting effects on cellulosis mi-crobes adherent to solid fractions in rumen of Xuanhan yellow cattle.关键词
菜籽粕/豆粕/棉籽粕/宣汉黄牛/纤维降解微生物Key words
rapeseed meal/soybean meal/cottonseed meal/Xuanhan yellow cattle/ruminal cellulosis mi-crobes分类
农业科技引用本文复制引用
吴小燕,彭全辉,王之盛,邹华围,陈慧,唐春梅..不同蛋白质饲料对宣汉黄牛瘤胃固相黏附纤维降解微生物数量的影响[J].动物营养学报,2014,(11):3298-3306,9.基金项目
国家肉牛牦牛产业技术体系（CARS-38）；农业部公益性行业专项饲料营养价值与畜禽饲养标准研究与应用 （）
