动物营养学报Issue(11)：3433-3442,10.DOI:10.3969/j.issn.1006-267x.2014.11.033
不同精粗比饲粮中添加壳聚糖对体外瘤胃发酵甲烷产量和发酵特性的影响
Effects of Chitosan Supplementation in Diets with Different Concentrate to Forage Ratios on Methane Production, and Fermentation Characteristics of in Vitro Ruminal Fermentation
摘要
Abstract
This experiment was conducted to investigate the effects of chitosan supplementation in diets with different concentrate to forage ratios on methane production, and fermentation characteristics of in vitro ruminal fermentation using long-term artificial rumen system-Rusitec system. A 2 × 2 two factors experimental design was adopted. The two factors were dietary concentrate to forage ratios [ 30∶70 ( low concentrate diet) and 70∶30 ( high concentrate diet) ] , and with or without chitosan supplementation ( 0 and 1 500 mg/tank) . Four groups with four replicates were composed. The results showed as follows: compared with the low concentrate diet, high concentrate diet significantly decreased methane production, neutral detergent fiber and crude protein degradation rates, as well as acetic acid and butyric acid contents, acetic acid to acrylic acid ratio and ammonia nitrogen concentration of fermentation fluid (P<0.05), and significantly increased total gas production and propionic acid content (P<0.05); the supplementation of chitosan significantly decreased methane and total gas production, crude protein degradation rate, as well as acetic acid and butyric acid contents, acetic acid to acrylic acid ratio and ammonia nitrogen concentration of fermentation fluid (P<0.05), and significantly in-creased propionic acid content ( P<0.05);there were interaction effects between concentrate to forage ratio and chitosan on methane and total gas production, crude protein degradation rate, ammonia nitrogen concentration of fermentation fluid at 0 and 6 h and acetic acid to acrylic acid ratio of fermentation fluid at 0 and 12 h ( P<0.05) , methane and total gas production, crude protein degradation rate and ammonia nitrogen concentration of fermentation fluid were decreased by the supplementation of chitosan in high concentrate diet at a bigger range than that in low concentrate diet, while total volatile fatty acid concentration and acetic acid to acrylic acid ratio were decreased by the supplementation of chitosan in high concentrate diet at a lower range than that in low concentrate diet. In conclusion, changing of dietary concentrate to forage ratio and chitosan supplemen-tation both can modify ruminal fermentation pattern, there is an interaction between the two factors, and the effects of chitosan supplementation in diets with 70∶30 of concentrate to forage ratio are better than those with 30∶70.关键词
壳聚糖/精粗比/甲烷/瘤胃发酵/Rusitec系统Key words
chitosan/concentrate to forage ratio/methane/ruminal fermentation/Rusitec system分类
农业科技引用本文复制引用
何玉鹏,郭艳丽,鞠九洲,秦士贞,郑琛..不同精粗比饲粮中添加壳聚糖对体外瘤胃发酵甲烷产量和发酵特性的影响[J].动物营养学报,2014,(11):3433-3442,10.基金项目
甘肃省农业科技创新项目（ GNCX-2012-44）；甘肃农业大学创新基金（ GAU-CX1039）；甘肃省草食动物生物技术重点实验室开放基金（ GKLAB201002）；甘肃省财政厅高校基本科研业务费项目 （）
