动物营养学报Issue(11)：3478-3484,7.DOI:10.3969/j.issn.1006-267x.2014.11.038
酵母培养物水溶物对离体草鱼肠道黏膜细胞生长及细胞膜完整性的影响
Effects of Water Soluble Material of Yeast Culture on Growth and Cell Membrane Integrity of Intestinal Epithelial Cells in Vitro of Grass Carp (Ctenopharyngodon idella)
摘要
Abstract
In this experiment, water soluble material of yeast culture as the experimental material was added in the culture solution of intestinal epithelial cells in vitro of grass carp ( Ctenopharyngodon idella) , in order to study the effects of water soluble material of yeast culture on growth and cell membrane integrity under the dif-ferent concentrations and different action time of water soluble material of yeast culture. One control group and 5 water soluble material of yeast culture groups ( YC-1 to 5 groups) were designed by using single-factor ex-perimental design, and each group had 96 replicates and 1 replicate was a culture hole. The control group was not added the water soluble material of yeast culture, and the concentration of water soluble material of yeast culture in YC-1 to 5 groups was 10, 25, 50, 100 and 200 mg/L, respectively. The results showed as follows:the cell morphology was no injury when adding 50 to 200 mg/L water soluble material of yeast culture into the culture solution, the cell activity was increased on 3 h after added 100 to 200 mg/L water soluble material of yeast culture, and the cell activity was increased on 6 h after added 50 mg/L water soluble material of yeast culture. Compared with control group, the activity of lactate dehydrogenase ( LDH) in culture solution in all water soluble material of yeast culture groups had no significant change on 3 h ( P>0.05) , while that in YC-4 and YC-5 groups was significantly reduced on 6 h ( P<0.05) , that in YC-3, YC-4 and YC-5 groups was sig-nificantly reduced on 9 h (P<0.05), and that in YC-2, YC-3, YC-4 and YC-5 groups was significantly re-duced on 12 h (P<0.05). The activities of glutamic-pyruvic transaminase (GPT) and glutamic-oxaloacetic transaminase ( GOT) in culture solution in all water soluble material of yeast culture groups had no significant change on all time points (3, 6, 9 and 12 h) compared with control group (P>0.05), but the activity of GOP on 6 to 9 h in all water soluble material of yeast culture groups was lower than that in control group. In conclu-sion, adding 10 to 200 mg/L water soluble material of yeast culture into the culture solution can improve the growth and protect the cell membrane integrity of intestinal epithelial cells in vitro of grass carp, and the appro-priate concentration of playing protective effect is 50 to 200 mg/L.关键词
草鱼/肠道黏膜细胞/酵母培养物水溶物/细胞生长/细胞膜完整性Key words
grass carp (Ctenopharyngodon idella)/intestinal epithelial cells/water soluble material of yeast culture/cell growth/cell membrane integrity分类
农业科技引用本文复制引用
姚仕彬,叶元土,蔡春芳,张宝彤,董娇娇,黄雨薇,陈科全..酵母培养物水溶物对离体草鱼肠道黏膜细胞生长及细胞膜完整性的影响[J].动物营养学报,2014,(11):3478-3484,7.基金项目
国家自然科学基金项目（31172417）；苏州市应用基础（农业）项目 （）
