动物营养学报Issue(12)：3616-3624,9.DOI:10.3969/j.issn.1006-267x.2014.12.010
外源寡糖对山羊瘤胃液酶活及粗饲料瘤胃降解率的影响
Effects of Exogenous Oligosaccharides on Enzymes Activities of Rumen Fluid and Degradation Rates of Roughages in Rumen of Laoshan Dairy Goats
摘要
Abstract
This experiment was conducted to study the effects of different oligosaccharides on main enzyme ac-tivities of rumen fluid and degradation rates of roughages in rumen of dairy goats. Six healthy mature castrated Laoshan dairy goats [(62.80±2.45) kg] with permanent cannulas were used. A 6×4 incomplete Latin square design was adopted. Goats were fed a basal diet ( control) and the basal diet supplemented with 1% mannanoli-gosaccharide ( MOS) , galacto-mannanoligosaccharides ( GMOS) , xylooligosaccharide ( XOS) , isomaltooli-gosaccharide ( IMO) and fructooligosaccharide ( FOS) , respectively. The trial lasted for 4 stages with 35 days per stage, and each stage included 25 days pre-test period before 10 days trial period. The results showed as follows:1) compared with the control group, MOS significantly increased the activity of carboxymethyl cellu-lase ( P<0.05) , GMOS, IMO and FOS significantly increased the activity of filter paper cellulase ( P<0.05) , XOS, IMO, FOS and GMOS significantly increased the activity of β-glucosidase ( P<0. 05 or P<0. 01 ) , MOS, GMOS, XOS, FOS and IMO significantly increased the activity of xylanase (P<0.05 or P<0.01), IMO, GMOS and FOS significantly increased the activity of neutral protease ( P<0.05) , and FOS significantly increased the activities of α-amylase (P<0.05). 2) Compared with the control group, MOS, GMOS, IMO XOS and FOS significantly improved the DM, ADF and NDF degradation rates of corn silage, peanut vine, chinensis and alfalfa in rumen. In conclusion, the optimal exogenous oligosaccharides were GMOS and FOS with the supplemental level of 1%.关键词
外源寡糖/山羊/瘤胃液/酶活/粗饲料/瘤胃降解率Key words
exogenous oligosaccharides/goats/rumen fluid/enzyme activity/roughages/ruminal degrada-tion rate分类
农业科技引用本文复制引用
林英庭,祁茹,王利华,朱风华,赵发盛,胡静..外源寡糖对山羊瘤胃液酶活及粗饲料瘤胃降解率的影响[J].动物营养学报,2014,(12):3616-3624,9.基金项目
山东省现代农业产业技术体系羊产业创新团队(SDAIT-09-011-04) （SDAIT-09-011-04）
青岛市科技计划应用基础研究项目(11-2-4-5-(1)-jch) （11-2-4-5-(1）
