动物营养学报Issue(12)：3689-3697,9.DOI:10.3969/j.issn.1006-267x.2014.12.019
猪血多肽对建鲤抗氧化能力和免疫功能的影响
Effects of Peptides from Swine Blood on Antioxidant Capacity and Immune Function of Jian Carp (Cyprinus carpio var. Jian)
摘要
Abstract
This experiment was conducted to study the effects of peptides from swine blood ( PSB) on antioxi-dant capacity and immune function of Jian carp ( Cyprinus carpio var. Jian) at in vitro and in vivo levels. In vitro experiment, the head-kidney macrophages and peripheral blood leukocytes, isolated from Jian carp, were cultured and treated for 24 hours with different concentrations (0, 10, 20, 40 and 80 μg/mL) of PSB. To e-valuate the immunostimulating effects of PSB, the viability of peripheral blood leukocytes was measured by WST-1 method, and the respiratory burst activities of oxygen and nitrogen of head kidney macrophages were investigated by nitroblue tetrazolium ( NBT) reduction method and Griess reagent colorimetric method, respec-tively. In vivo experiment, PSB was added to the basal diet at the concentrations of 0, 0. 5, 1. 0, 2. 0 and 5.0 g/kg, fed to Jian carp in constant temperature cycle water system for 8 weeks. The blood samples were collected four times around the time points of the 1st week, the 2nd week, the 4th week and the 8th week, then detected the changes of serum biochemical indices, antioxidant indices and immune indices. The results showed as follows: in vitro experiment, the PSB at the concentrations of 20, 40 and 80 μg/mL could signifi-cantly enhance the viability of peripheral blood leucocytes ( P<0.05 or P<0.01); PSB at the concentrations of 40 and 80 μg/mL could significantly enhance the respiratory burst activities of oxygen and nitrogen ( P<0.05 or P<0.01) . In vivo experiment, after Jian carp were fed the diet with PSB at the concentration of 5.0 g/kg, the contents/activity of 3 biochemical indices [ albumin ( ALB) , total protein ( TP) and alkaline phosphatase ( AKP) ] , the activities of 3 antioxidant enzymes [ superoxide dismutase ( SOD) , catalase ( CAT) and gluta-thione peroxidase ( GSH-Px) ] and the contents of 3 immune factors [ interleukin-1β ( IL-1β) , tumor necrosis factor-α ( TNF-α) and complement 4 ( C4) ] in serum at the 4th week and the 8th week were significantly in-creased ( P<0.05); moreover, the content of complement 4 ( C4) in serum at the 8th week was significantly increased aslo ( P<0.05) . Therefor, the PSB can improve the antioxidant capacity and immune function of Jian carp.关键词
猪血多肽/抗氧化能力/免疫功能/建鲤Key words
peptides from swine blood/antioxidant capacity/immune function/Jian carp ( Cyprinus carpio var. Jian)分类
农业科技引用本文复制引用
王加豪,贾睿,刘英娟,杜金梁,曹丽萍,殷国俊..猪血多肽对建鲤抗氧化能力和免疫功能的影响[J].动物营养学报,2014,(12):3689-3697,9.基金项目
中央级公益性科研院所基本科研业务费专项资金(2013JBFM11,2013JBFM12) （2013JBFM11,2013JBFM12）
国家自然科学基金青年科学基金(31202002,31200918) （31202002,31200918）
江苏省自然科学基金( BK2012535) （ BK2012535）
