动物营养学报Issue(1)：154-164,11.DOI:10.3969/j.issn.1006-267x.2015.01.019
鱼粉蛋白与发酵酶解豆粕蛋白不同配比对日本沼虾生长及免疫性能的影响
Effects of Different Protein Ratios of Fish Meal to Fermented and Enzymolysis Soybean Meal on Growth and Immune Performance of Oriental River Prawn (Macrobrachium nipponense)
摘要
Abstract
This experiment was conducted to evalute the effects of different protein ratios of fish meal to fer-mented and enzymolysis soybean meal with microencapsulated amino acid supplementation in diets on growth and immune performance of oriental river prawn ( Macrobrachium nipponense ) . Fermented enzymolysis soy-bean meal replaced 0 ( FM group) , 25% ( R25 group) , 50% ( R50 group) , 75% ( R75 group) and 100% of fish meal ( R100 group) ( the protein ratios of fish meal to fermented and enzymolysis soybean meal were 1∶0, 3 ∶1, 1∶1, 1∶3 and 0∶1, respectively) in five isocaloric and isonitrogenous diets, respectively. The oriental river prawn with the average body weight of (0.103 0±0.000 2) g were randomly divided into 5 groups with 5 rep-licates in each group and 50 prawns in each replicate. The experiment lasted for 8 weeks. After the feeding ex-periment, the prawns were challenged with Aeromonas hydrophila. The results showed that weight gain rate and specific growth rate were the highest when the protein ratio of fish meal to fermented and enzymolysis soy-bean meal was 1∶1, and were significantly higher than those in the FM and R100 groups ( P<0.05) , while no significant difference was observed in survival ratio among all groups (P>0.05). There were no significant differences in the activities of superoxide dismutase ( SOD) and glutathione peroxidase ( GSH-Px) and the con-tent of malondialdehyde (MDA) in hepatopancreas among all groups (P>0.05). The total haemocyte count ( THC) and haemolymph phagocytic activity in the FM group were significantly higher than those in the R100 group ( P<0.05) . The hepatopancreas heat shock cognate protein ( HSC70) mRNA relative expression level in the FM group was the highest, and significantly higher than that in the other groups (P<0.05), the same of heat shock protein 90 ( HSP90) in R25 group ( P<0.05) . Additionally, the cumulative mortality was signifi-cantly increased when the ratio of fish meal replacement with fermented and enzymolysis soybean meal was more than 50% after challenged with Aeromonas hydrophila. Therefore, fermented and enzymolysis soybean meal can be an appropriate protein source in the diet of oriental river prawn, and the optimal dietary protein ra-tio of fish meal to fermented and enzymolysis soybean meal for oriental river prawn is 1∶1 under the condition of amino acid balance.关键词
日本沼虾/发酵酶解豆粕/鱼粉/生长性能/免疫力Key words
oriental river prawn ( Macrobrachium nipponense )/fermented and enzymolysis soybean meal/fish meal/growth performance/immunity分类
农业科技引用本文复制引用
丁志丽,张易祥,叶金云,周志金,杜震宇..鱼粉蛋白与发酵酶解豆粕蛋白不同配比对日本沼虾生长及免疫性能的影响[J].动物营养学报,2015,(1):154-164,11.基金项目
浙江省重大科技专项计划项目(2014C12SA560016) （2014C12SA560016）
浙江省科技计划项目(2012C12008-2) （2012C12008-2）
浙江省自然科学基金(LQ14C190004) （LQ14C190004）
国家自然科学基金(31402308) （31402308）
上海市农业委员会虾类产业技术体系建设项目 （）
