动物营养学报Issue(1)：171-177,7.DOI:10.3969/j.issn.1006-267x.2015.01.021
用高效液相色谱法检测鸡胚组织基因组DNA甲基化水平变化规律
Analysis of Variation of Genome DNA Methylation Level of Chick Embryo Tissues with High Performance Liquid Chromatography
摘要
Abstract
This experiment was conducted to develop the high performance liquid chromatography ( HPLC ) method for genome DNA methylation level detection, and to explore the variation of genome DNA methylation level of chick embryo tissues preliminary. Firstly, the genome DNA methylation level detection method was improved from 2 aspects including DNA enzymatic hydrolysis system and HPLC instrument detection condi-tion; then, the DNA methylation level of heart, liver and muscle at embryonic ages of 8, 11, 14, 17 days of Cobb 500 broiler chick embryo was detected by improved HPLC method, respectively. The results showed that the genome DNA methylation level of 3 tissues was increased with embryonic age increasing and that of liver was significantly higher than that of heart and muscle in later embryonic development ( embryonic age of 17 days) ( P<0.05) . Heart and muscle showed no significant difference in DNA methylation level among all em-bryonic ages (P>0.05). It is concluded that the developed HPLC laid a good foundation for genome DNA methylation determination of tissues rapidly and accurately. Otherwise, the genome DNA methylation level ri-ses gradually with chick embryonic age increasing, and that of liver is higher in later embryonic development.关键词
高效液相色谱/肉鸡/胚胎/DNA甲基化Key words
HPLC/chick/embryo/DNA methylation分类
农业科技引用本文复制引用
李世召,郭伟,杜超,景宇飞,刘艳利,申静,姚军虎,杨小军..用高效液相色谱法检测鸡胚组织基因组DNA甲基化水平变化规律[J].动物营养学报,2015,(1):171-177,7.基金项目
国家自然基金项目(31272464,31001017) （31272464,31001017）
教育部新世纪优秀人才(NCET-12-0476) （NCET-12-0476）
陕西省科技新星基金(2012KJXX-18) （2012KJXX-18）
