动物营养学报Issue(1)：313-319,7.DOI:10.3969/j.issn.1006-267x.2015.01.038
蚕沙和稻秸不同比例组合对瘤胃微生物体外发酵的组合效应
Associative Effects of Different Combination Ratios of Silkworm Excrement and Rice Straw on the Rumen Microbial Fermentation in Vitro
摘要
Abstract
In vitro fermentation was conducted to investigate associative effects of different combination ratios of silkworm excrement ( SE) and rice straw ( RS) . SE and RS were mixed at the ratios of 100∶0 ( SE100 group) , 80∶20 ( SE80 group) , 60∶40 ( SE60 group) , 40∶60 ( SE40 group) , 20∶80 ( SE20 group) and 0∶100 ( SE0 group) , respectively. The mixtures were incubated in vitro for 24 hours for a batched in vitro fermenta-tion test and 72 hours for an in vitro fermentation gas production test. Gas production parameters and fermenta-tion characteristics were determined. The results showed as follows:1) theological maximum gas production in SE100 group was significantly higher than that of the other groups ( P<0.05) , and accumulated gas production of SE80 group was significantly higher than that of the other groups (P<0.05). 2) Total volatile fatty acid concentration in culture medium at 24 hours showed SE80 group>SE100 group>SE60 group>SE40 group>SE0 group>SE20 group, and acetate/propionate showed SE80 group<SE40 group<SE20 group<SE60 group<SE0 group<SE100 group. 3) Microbial protein concentration in culture medium at 24 hours showed SE20 group>SE100 group>SE60 group>SE80 group>SE40 group>SE0 group. 4) With the increasing of SE ratio, in vitro digestion rate of organic matter of substrate was increased. 5) Multiply factor associative effect index of SE80 group was 0.76, and showed SE80 group>SE40 group>SE60 group>SE20 group. In conclusion, the combina-tion of SE and RS can improve in vitro fermentation characteristics and gas production parameters, and the op-timal ratio of SE and RS is 80∶20.关键词
蚕沙/稻秸/体外发酵/组合效应Key words
silkworm excrement/rice straw/in vitro fermentation/associative effect分类
农业科技引用本文复制引用
孙丽莎,李华伟,崔慧慧,赵睿,王梦芝,喻礼怀,王洪荣..蚕沙和稻秸不同比例组合对瘤胃微生物体外发酵的组合效应[J].动物营养学报,2015,(1):313-319,7.基金项目
国家公益性行业(农业)科研专项“南方地区肉羊育肥与高品质肉生产技术研究”(201303144) （农业）
扬州大学动物营养与饲料科学优势学科专项 （）
江苏省科技支撑计划( BE2013393) （ BE2013393）
