动物营养学报Issue(2)：638-646,9.DOI:10.3969/j.issn.1006-267x.2015.02.037
免疫增强剂党参对仿刺参肠道菌群结构的影响
Effect of Codonopsis pilosula as an Immunopotentiator on Intestinal Bacterial Community Composition of Apostichopus japonicus
摘要
Abstract
By plate counting method and polymerase chain reaction ( PCR)-denaturing gradient gel electropho-resis ( DGGE ) technology, the effect of Codonopsis pilosula as an immunopotentiator on intestinal bacterial community composition of Apostichopus japonicas was investigated. Apostichopus japonicas with the initial body weight of (18.00±2.00) g were randomly divided into 2 groups (control group and experimental group) with 6 replicates per group and 12 Apostichopus japonicas per replicate. Apostichopus japonicas in the control group were fed a diet which were formulated using sea mud and Sargassum thunbergii powder with the mass ra-tio of sea mud to Sargassum thunbergii ratio was 1∶1, while those in the experimental group were fed the diet adding Codonopsis pilosula with the quantity of 2% Sargassum thunbergii powder weight. The feeding trial las-ted for 28 days. The results showed that immunopotentiator-Codonopsis pilosula could significantly promote the special growth rate ( P<0.05) , reduce the feed conversion ratio ( P<0.05) , and increase the count of hetero-trophic in intestinal content of Apostichopus japonicas (P<0.05). Sequence statistics showed that the propor-tion of superior sequence was significantly increased after feeding Codonopsis pilosula ( P<0.05) , which in ex-perimental group was 97.57% and that in control group was only 80.22%. Beta-diversity analysis reported that the intestinal microenvironment of Apostichopus japonicas was changed after feeding Codonopsis pilosula, and the rages of coefficient variation were 14.91% to 15.47%, 15.47% to 16.21%, and 14.91% to 16.21%. A-bundance analysis showed that the abundances of Proteobacteria and Bacteroidetes were increased, but the a-bundances of Verrucomicrobia, Actinobacteria and Firmiaites were decreased after feeding Codonopsis pilosula. The similarity coefficient of intestinal bacterial community composition between control group and experimental group was 0.97 by cluster analysis. It is concluded that Codonopsis pilosula as an immunopotentiator can im-prove the specific growth rate, reduce the feed conversion ratio, increase the count of heterotroph and the a-bundance of dominant bacteria in intestine, and optimize the intestinal microenvironment of Apostichopus ja-ponicus.关键词
免疫增强剂/党参/仿刺参/肠道/菌群/DGGEKey words
immunopotentiator/Codonopsis pilosula/Apostichopus japonicus/intestine/bacterial communi-ty/DGGE分类
农业科技引用本文复制引用
樊英,李乐,于晓清,刘恩孚,李天保,许拉,叶海斌..免疫增强剂党参对仿刺参肠道菌群结构的影响[J].动物营养学报,2015,(2):638-646,9.基金项目
山东省现代农业产业技术体系刺参产业创新团队建设项目(SDAIT-08) （SDAIT-08）
山东省科技发展计划项目(2012YD10016) （2012YD10016）
海洋公益性行业科研专项经费项目(201305005) （201305005）
“十二五”科技支撑计划项目(2011BAD13B03) （2011BAD13B03）
