动物营养学报Issue(3)：997-1004,8.DOI:10.3969/j.issn.1006-267x.2015.03.041
饲粮阴阳离子差水平对围产期小鼠血钙浓度与胃肠道组织维生素 D受体 mRNA表达水平的影响
Effects of Dietary Cation-Anion Difference Level on Plasma Calcium Concentration and Gastrointestinal Tract Vitamin D Receptor mRNA Expression Level of Mice during the Transition Period
摘要
Abstract
This experiment was conducted to study the effects of dietary cation-anion difference ( DCAD) level on plasma calcium concentration and gastrointestinal tract vitamin D receptor ( VDR ) mRNA expression level of mice during the transition period, and to exposit the mechanism of low DCAD level control of hypocalce-mia. One hundred and twenty mice during the transition period were randomly allocated into 3 groups with 40 mice in each group, mice in the 3 groups were fed 3 diets with DCAD level at+300 ( high DCAD level group, HD group) , +150 ( control group, CON group) , and -150 ( low DCAD level group, LD group) , respec-tively. Ten mice for each group were sacrificed to collect blood sample for plasma calcium concentration analy-sis and gastrointestinal tract tissues ( stomach, duodenum, jejunum, ileum, cecum, and colon) samples for VDR mRNA expression level detection at 4 time points:day of 20 (-20 d) and 5 (-5 d) before kidding, day of kidding ( 0 d) , and day of 3 after kidding (+3 d) . The results showed that the plasma calcium concentra-tion in LD group was higher than that in HD and CON groups for the whole and individual blood sampling time ( P<0.05) . At-20 d, compared with HD and CON groups, the VDR mRNA expression level in jejunum, ile-um and colon in LD group was significantly increased ( P<0.05); at -5 d, the VDR mRNA expression level in jejunum and colon in LD group was significantly increased ( P<0.05); at 0 d, the VDR mRNA expression level in duodenum in LD group was significantly increased ( P<0.05); at +3 d, the VDR mRNA expression level in duodenum, jejunum and colon in LD group was significantly increased ( P<0.05) . The results indica-ted that low DCAD level can increase VDR mRNA expression level in gastrointestinal tract of mice during the transition period, and increase plasma calcium concentration, which was probably the mechanism of low DCAD preventing hypocalcemia.关键词
饲粮阴阳离子差/血钙/维生素D受体/围产期小鼠Key words
dietary cation-anion difference/plasma calcium/vitamin D receptor/mice during the transition period分类
农业科技引用本文复制引用
莘海亮,吴文旋,杨艺,段永邦,张吉鹍,朱伦琴,田兴舟,李存刚,汤志宏,刘明雪..饲粮阴阳离子差水平对围产期小鼠血钙浓度与胃肠道组织维生素 D受体 mRNA表达水平的影响[J].动物营养学报,2015,(3):997-1004,8.基金项目
国家自然科学基金(31160468 （）
31360563) （）
