动物营养学报Issue(4)：1145-1151,7.DOI:10.3969/j.issn.1006-267x.2015.04.018
饲料中维生素 D3添加水平对黄鳝生长性能及免疫功能的影响
Effects of Vitamin D3 Supplemental Level on Growth Performance and Immune Function of Monopterus albus
摘要
Abstract
This experiment was conducted to study the effects of vitamin D3 supplemental level on growth per⁃formance and immune function of Monopterus albus. A total of 540 healthy M. albus with an average body weight of (21.7±2.1) g was randomly assigned to 6 groups with 3 replicates per group and 30 M. albus per replicate. The M. albus in the 6 groups were fed with the basal diet supplemented with 0 (control group), 250, 500, 1 000, 2 000 and 4 000 IU/kg vitamin D3, respectively. After 90 days feeding, researchers ran⁃domly selected 6 M. albus in each group, and drew blood from caudal vein to detect the related immune inde⁃xes of peripheral blood after growth performance determination. Furthermore, researchers injected Aeromonas hydrophila into 20 M. albus randomly selected in each group to observe the cumulative mortality of the M. al⁃bus challenged with A. hydrophila. The results showed as follows: compared with the control group, except 4 000 IU/kg group, the weight gain rate and protein efficiency ratio in other groups were significantly im⁃proved ( P<0.05) , and feed conversion ratio was significantly decreased ( P<0.05); in 500 and 1 000 IU/kg groups, the levels of immunoglobulin M, complement 3 and complement 4, the activity of lysozyme, and the amounts of CD3, CD4 and CD8 of T cells with CD4/CD8 in serum were increased significantly ( P<0.05) , in addition the number of whole blood leukocyte, neutrophil, intermediate cell and lymphocyte was significant⁃ly increased (P<0.05), moreover, the cumulative mortality of the M. albus challenged with A. hydrophila was obviously decreased. No significant differences were found in all indexes except serum immunoglobulin M level between 4 000 IU/kg group and control group ( P>0.05) . In conclusion, supplementation of appropriate ( 500 and 1 000 IU/kg) vitamin D3 can enhance humoral immunity, cellular immunity and nonspecific immu⁃nity function of M. albus, thus improving the resistance ability to pathogenic bacterium;moreover, lack or ex⁃cessive supplementation of vitamin D3 can result in the lower immune function of M. albus.关键词
黄鳝/维生素D3/生长性能/免疫功能Key words
Monopterus albus/vitamin D3/growth performance/immune function分类
农业科技引用本文复制引用
黎德兵,邵珊珊,张龚炜,周定刚,李学伟..饲料中维生素 D3添加水平对黄鳝生长性能及免疫功能的影响[J].动物营养学报,2015,(4):1145-1151,7.基金项目
四川省教育厅自然科学重点项目 （）
