动物营养学报Issue(4)：1243-1251,9.DOI:10.3969/j.issn.1006-267x.2015.04.031
饲粮物理有效中性洗涤纤维水平对8～10月龄奶牛瘤胃发酵参数和纤维降解菌的影响
Effects of Dietary Physically Effective Neutral Detergent Fiber Level on Rumen Fermentation Parameters and Cellulolytic Bacteria of 8 to 10-Month-Old Heifers
摘要
Abstract
The objective of this experiment was to evaluate effects of dietary physically effective neutral deter⁃gent fiber ( peNDF) level on rumen fermentation parameters and cellulolytic bacteria of 8 to 10⁃month⁃old heif⁃ers. Twenty⁃four Chinese Holstein heifers with similar month of age and body weight were randomly divided into 4 groups. Heifers were offered one of four diets, which were chemically identical but included different peNDF levels [22.73%, 23.92%, 25.85% and 28.39%] through cutting the roughage of Chinese wildrye into different lengths. The pre⁃test lasted for 10 days, and the test lasted for 60 days. The results showed as fol⁃lows:1) the intakes of dry matter, neutral detergent fiber and acid detergent fiber of heifers were not signifi⁃cantly affected by peNDF level (P>0.05). 2) Rumen fluid pH increased with the increasing peNDF level, 22.73% group was significantly lower than the other 3 groups ( P<0.01);there were no effects of peNDF lev⁃el on total volatile fatty acid concentration, and acetate and butyrate contents ( P>0.05) , however, with the in⁃creasing of peNDF level, propionate content decreased, and 22. 73% group was significantly higher than 23.92% group (P<0.01) and 28.39% group (P<0.05); the ratio of acetate to propionate of 23.92% group was significantly higher than that of 22.73% group ( P<0.01) and 25.85% group( P<0.05);ammonia⁃N con⁃centration of 25.85% group was significantly higher than that of the other 3 groups ( P<0.01 or P<0.01) . 3) Using 22.73% group as control, the relative content of Fibrobacter succinogene of the other 3 groups was in⁃creased with increasing peNDF level, and that of 28. 39% group was the highest, but was still significantly lower than that of 22.73% group ( P<0.01);with the increasing of peNDF level, the relative content of Rumi⁃nococcus flave faciensk, Ruminococcus Albus and Prevotella rumicola increased initially and then decreased, and the highest contents all appeared in 25.85% group, which were significantly higher than those of the other 3 groups ( P<0.05 or P<0.01) . It is concluded that properly increasing of dietary peNDF level is beneficial for improvement of rumen fluid pH and rumen fermentation, as well as the content of cellulolytic bacteria in ru⁃men; under the conditions in the present study, 25. 85% is the optimal peNDF1.18 level in diets for 8 to 10⁃month⁃old heifers.关键词
物理有效中性洗涤纤维/采食量/瘤胃液pH/氨态氮/挥发性脂肪酸/纤维降解菌/后备奶牛Key words
peNDF/feed intake/rumen fluid pH/ammonia nitrogen/volatile fatty acid/cellulolytic bacteri-a/heifers分类
农业科技引用本文复制引用
陈青,王洪荣,葛汝方,崔慧慧,王梦芝,喻礼怀,林淼..饲粮物理有效中性洗涤纤维水平对8～10月龄奶牛瘤胃发酵参数和纤维降解菌的影响[J].动物营养学报,2015,(4):1243-1251,9.基金项目
国家“十二五”科技支撑项目（2012BAD12B02-2）；江苏高校优势学科建设工程资助项目 （）
