动物营养学报Issue(4)：1278-1292,15.DOI:10.3969/j.issn.1006-267x.2015.04.035
花鲈和西伯利亚鲟生长激素／类胰岛素样生长因子－Ⅰ轴相关基因全长 cDNA的克隆和序列分析
Cloning and Sequence Analysis of Full Length cDNAs of Growth Hormone/Insulin-Like Growth Factor-Ⅰ Axis Relative Genes of Japanese Seabass (Lateolabrax japonicus) and Siberian Sturgeon (Acipenser baerii Brandt)
摘要
Abstract
The reverse transcription⁃phosphatase chain reaction ( RT⁃PCR ) and rapid amplification of cDNA ends ( RACE) methods were used in this experiment to clone the full⁃length sequences of cDNAs for growth hormone ( GH) and GH receptor ( GHR) of Japanese seabass ( Lateolabrax japonicus) and Siberian sturgeon ( Acipenser baerii Brandt ) . The results showed as follows: the full⁃length GH cDNA of Japanese seabass ( GenBank accession No. JQ995145) was 949 bp, consisting of an open reading frame ( ORF) of 615 bp en⁃coding a protein of 204 amino acids with a theoretical molecular weight of 23. 06 ku, and shared 36. 0% to 90.2% similarity with other species. The full⁃length GHRⅠ cDNA of Japanese seabass ( GenBank accession No. JX402001) was 3 070 bp, consisting of an ORF of 1 911 bp encoding a protein of 637 amino acids with a theoretical molecular weight of 70.79 ku, and shared 32.2% to 86.2% similarity with other species. The full⁃length GHRⅡcDNA of Japanese seabass ( GenBank accession No. JQ995146) was 2 926 bp, consisting of an ORF of 1 749 bp encoding a protein of 582 amino acids with a theoretical molecular weight of 64.42 ku, and shared 30.8% to 81. 4% similarity with other species. The full⁃length GH cDNA of Siberian sturgeon ( Gen⁃Bank accession No. JX003684) was 999 bp, consisting of an ORF of 645 bp encoding a protein of 214 amino acids with a theoretical molecular weight of 24.14 ku, and shared 38.3% to 70.1% similarity with other spe⁃cies. The full⁃length GHR cDNA of Siberian sturgeon ( GenBank accession No. JX003685 ) was 2 238 bp, consisting of an ORF of 1 716 bp encoding a protein of 571 amino acids with a theoretical molecular weight of 63.41 ku, and shared 39. 5% to 49. 2% with other species. The full⁃length sequences of cDNAs for GH and GHR of Japanese seabass and Siberian sturgeon will provide a scientific basis for further study on the regulatory mechanism of GH/IGF⁃Ⅰ axis on growth and development of fish.关键词
花鲈/西伯利亚鲟/基因克隆/GH/IGF-Ⅰ轴/生长激素/生长激素受体Key words
Japanese seabass ( Lateolabrax japonicus)/Siberian sturgeon ( Acipenser baerii Brandt)/gene cloning/GH/IGF-Ⅰ axis/growth hormone/growth hormone receptor分类
农业科技引用本文复制引用
张志勇,薛敏,王嘉,吴秀峰,郑银桦,韩芳..花鲈和西伯利亚鲟生长激素／类胰岛素样生长因子－Ⅰ轴相关基因全长 cDNA的克隆和序列分析[J].动物营养学报,2015,(4):1278-1292,15.基金项目
国家自然科学基金（31101907，31372539）；公益性行业（农业）专项经费项目（201203015）；北京市现代农业产业技术体系 （）
