动物营养学报2015，Vol.27Issue(5)：1431-1439,9.DOI:10.3969/j.issn.1006-267x.2015.05.013
饲料中维生素 E水平对三疣梭子蟹幼蟹生长性能、血清非特异性免疫及抗氧化指标的影响
Effects of Dietary Vitamin E Level on Growth Performance, Serum Non?Specific Immune and Anti?Oxidant Indices of Juvenile Swimming Crab ( Portunus tribueculatus)
摘要
Abstract
An 8?week feeding trial was conducted to evaluate the effects of dietary vitamin E level on growth performance, serum non?specific immune anti?oxidant indices of juvenile swimming crab ( Portunus tribuecula?tus). Juvenile swimming crab with an average body weight about of (5.94±0.17) g were used as test object. Six iso?nitrogenous and iso?energetic diets were formulated to contain 8. 9, 19. 6, 27. 0, 39. 7, 77. 6 and 156.9 mg/kg vitamin E, respectively. Each experimental diet fed 60 crab, and per 20 crab as a replicate. Re?sults showed that weight gain rate ( WGR) and specific growth rate ( SGR) were significantly increased with dietary vitamin E level increasing from 8.9 to 39.7 mg/kg ( P<0.05);however, WGR and SGR showed a de?crease trend when dietary vitamin E level exceeded 39.7 mg/kg ( P>0.05) . Dietary vitamin E level had no sig?nificant effects on the feed conversion ratio, survival rate and molting rate of juvenile crab among all groups ( P>0.05) . Crude protein, moisture and ash contents in whole body and total protein, triglyceride and glucose contents in serum were not significantly affected by dietary vitamin E level ( P>0.05) , while crude lipid con?tent in whole body was significantly influenced by dietary vitamin E level (P<0.05). Lysozyme and alkaline phosphatase activities in serum were firstly increased and then decreased with dietary vitamin E level increas?ing, and the highest activities of lysozyme and alkaline phosphatase activities in serum were found when dietary vitamin E level was 39. 7 mg/kg. Dietary vitamin E level had no significant effect on serum superoxide dis?mutase (SOD) activity (P>0.05), however, serum malondialdehyde (MDA) content was gradually de?creased with dietary vitamin E level increasing, and crabs fed the diet containing 156.9 mg/kg vitamin E had the lowest serum MDA content which significantly lower than that in 8.9 mg/kg group (P<0.05). Based on broken?line regression analysis of the SGR, the optimal vitamin E requirement is estimated to be 39.0 mg/kg (dry weight of diet) for juvenile swimming crab.关键词
三疣梭子蟹幼蟹/维生素E/生长性能/抗氧化指标/非特异性免疫指标Key words
juvenile swimming crab/vitamin E/growth performance/anti?oxidant indices/non?specific im?mune indices分类
农业科技引用本文复制引用
李弋,张稳,金敏,霍雅文,邱红,侯迎梅,周歧存..饲料中维生素 E水平对三疣梭子蟹幼蟹生长性能、血清非特异性免疫及抗氧化指标的影响[J].动物营养学报,2015,27(5):1431-1439,9.基金项目
国家自然科学基金项目(41476125) （41476125）
宁波市海洋蟹类产业科技创新团队项目(2011B81003) （2011B81003）
