动物营养学报2015，Vol.27Issue(5)：1586-1595,10.DOI:10.3969/j.issn.1006-267x.2015.05.031
硝酸钠和2-溴乙烷磺酸钠对山羊体外瘤胃发酵甲烷、氢气和挥发性脂肪酸生成的影响
Effect of Sodium Nitrate and 2?Bromoethanesulphonate on Methane, Hydrogen and Volatile Fatty Acids Production of in Vitro Ruminal Fermentation
摘要
Abstract
The aim of this study was to investigate the mechanism of sodium nitrate (NaNO3) and 2?bromoeth?anesulphonate ( BES) on methane inhibition from the biochemical aspect of ruminal hydrogen metabolism. Ru?men fluid was collected from two adult Xiangdong black goats with permanent rumen fistulas. The study con?tained control group, NaNO3 groups ( supplemental level was 0.10, 0.20 and 0.40 mg/mL, respectively) and BES groups (supplemental level was 0.63, 1.26 and 2.52 mg/mL, respectively). The 24 h in vitro ruminal incubation was performed to obtain gas production, production and contents of hydrogen and methane, and volatile fatty acids production and composition using automatic fermentation equipment. The results showed as follows: NaNO3 and BES supplementation significantly lowered methane production in comparison with control group ( P<0.05) , and increasing supplemental level of NaNO3 and BES linearly decreased methane production ( P<0.05) . The lower supplemental level of NaNO3(≤0.20 mg/mL) had no significant effects on gas produc?tion, initial fractional rate of degradation (FRD0), acetate and propionate production and the ratio of acetate to propionate (P>0.05), while the higher supplemental level of NaNO3(0.40 mg/mL) had significantly higher hydrogen production ( P<0.05) , and significantly lower gas production, methane production, FRD0 and total volatile fatty acids production in comparison with control group ( P<0.05) . BES had no significant effects on hydrogen production, FRD0 and total volatile fatty acids production in comparison with control group ( P>0.05), but had significantly higher hydrogen production and content (P<0.05), and decreased the ratio of ac?etate to propionate (P<0.05). BES could alter pattern of rumen fermentation through facilitating propionate production. In conclusion, NaNO3 reduces methane production through hydrogen sink and rumen microbial tox?icity, while BES reduced methane production through inhibiting the activity of methanogens, and had little effects on gas production and substrate degradation.关键词
体外瘤胃发酵/发酵类型/甲烷减排/甲烷抑制剂/硝酸盐还原菌Key words
in vitro ruminal fermentation/fermentation pattern/methane reduction/methane inhibitors/ni?trate reducing bacteria分类
农业科技引用本文复制引用
王荣,谭利伟,王敏,邓近平,颜志成,王玉诗,谭支良..硝酸钠和2-溴乙烷磺酸钠对山羊体外瘤胃发酵甲烷、氢气和挥发性脂肪酸生成的影响[J].动物营养学报,2015,27(5):1586-1595,10.基金项目
国家自然科学基金( 31472133 ) （ 31472133 ）
中国科学院战略性先导科技专项( XDA05020700 ) （ XDA05020700 ）
"十二五"国家科技支撑计划项目(2012BAD14B17,2012BAD12B02) （2012BAD14B17,2012BAD12B02）
中央驻湘科研机构技术创新发展专项(2013TF3006) （2013TF3006）
