安亚南 1王丹阳 1丁立人 1杨新岗 1邓亚军 1刘强1
作者信息
- 1. 南京农业大学动物科技学院，南京 210095
- 折叠
摘要
Abstract
This experiment was conducted to develop a high-performance liquid chromatographic ( HPLC ) method for quantitative determination of aflatoxin B1 and zearalenone in supernatant liquor of gastric and intesti-nal of pigs, and the method was used to evaluate binding efficacy of aflatoxin B1 and zearalenone by mycotoxin absorbents in different media. In order to eliminate the influence of complex composition in the supernatant, the experiment optimized extraction and purification of the supernatant, detection wavelengths and mobile phases of liquid chromatography. Binding efficacy of 4 mycotoxin adsorbents in a fixed mycotoxin concentration was e-valuated respectively in purified water,the supernatant liquor of gastric and intestinal chyme of pigs. The results showed as follows:1) the supernatant liquor samples were extracted with n-hexane and dichloromethane, the optimal chromatographic condition was that the fluorescent emitted light and excitation light wavelength were set as 360, 440 nm within 0 to 9 minutes and 235, 418 nm within 9 to 14 minutes, respectively, and the mo-bile phase was the mixture of acetonitrile and water with acetonitrile percentage in the range of 40% to 60%. The analytical method was quantitative determination for aflatoxin B1 from 1. 00 to 10 000. 00 ng/mL and zearalenone from 20.00 to 5 000.00 ng/mL, respectively. The linear correlation coefficient of aflatoxin B1 was 1; the limit of detection was 0.16 ng/mL, the intra-day and inter-day precision were 2.37% and 2.74%, with recoveries ranging from 89. 1% to 100. 3%. The linear correlation coefficient of zearalenone was 0. 999; the limit of detection was 2.00 ng/mL, the intra-day and inter-day precision were 4.50% and 8.84%, with recov-ery ranging from 98. 7% to 104. 1%. 2) The adsorption rates of aflatoxin B1 on P3, M3, Y1 and Y2 were 72.10%, 84.18%, 83.17% and 66.01% from gastric supernatant of pigs, and 78.15%, 88.08%, 88.12% and 67.34% from intestinal supernatant,respectively. The binding rate of zearalenone on P3, M3, Y1 and Y2 were 85.35%, 18.41% and 16.20% from gastric supernatant of pigs, and 12.98%, 39.75%, 25.15%, 21.40% and 24.85% from intestinal supernatant,respectively. In conclusion, the HPLC method is developed for simultane-ous measurement of aflatoxin B1 and zearalenone in supernatant liquor of gastric and intestinal chyme of pigs, and its detection limits and ranges meet the requirement of Hygienical Standard for Feeds in our country. The method is applied to evaluate binding efficacy of mycotoxin adsorbents under the condition of physiological body fluid of pigs.关键词
黄曲霉毒素B1/玉米赤霉烯酮/霉菌毒素吸附剂/猪食糜上清液/吸附效果Key words
aflatoxin B1/zearalenone/mycotoxin adsorbent/supernatant liquor of chyme of pigs/binding ef-ficacy分类
农业科技
