动物营养学报Issue(6)：1921-1927,7.DOI:10.3969/j.issn.1006-267x.2015.06.031
山羊瘤胃中单宁降解菌的分离、筛选和鉴定
LI Xingfan1 XU Wen1 ZHANG Shengnan1 XU Gaoqiao2 WANG Shanru1 WANG Shichang1 LIANG Shizhong1
摘要
Abstract
This study was conducted to isolate, screen and identify several strains of tannin degradable bacteria in rumen of goats. Ruminal contents was collected from goats, and were preliminary screened by tolerance cul-ture medium with different tannic concentrations, then strains of tannin degradable bacteria were isolated, screened by tannase identification culture mediums. The screened strains were firstly identified by the methods of 16S rDNA gene sequencing, and then were cultured on tannase inductive culture mediums with different tan-nin concentrations ( 0. 5%, 1% and 1. 5%, respectively ) for the analysis of enzyme activity. The results showed as follows: four strains of tannin degradable bacteria were isolated and screened. According to the re-sults of 16S rDNA gene sequencing, the strains were Providencia alcalifaciens, Kerstersia, Providencia Ver-micola and Pseudomonas aeruginosa, respectively. After cultured on tannase inductive culture medium, the in-tra-cellular tannase activity of Kerstersia was higher than that of the other three strains, which was pretty close to each other; after inducted by 1.5% tannin, the activity was significantly higher than that inducted by 0.5%and 1% tannin ( P<0.05) . In conclusion, four strains with high enzyme activity of tannin degradable bacteria in rumen of goats was successfully isolated, screened and identified.关键词
单宁降解菌/瘤胃/筛选/鉴定Key words
tannin degradable bacteria/rumen/screening/identification分类
农业科技引用本文复制引用
李兴芳,徐雯,张胜男,徐高跷,汪珊如,王士长,梁世忠..山羊瘤胃中单宁降解菌的分离、筛选和鉴定[J].动物营养学报,2015,(6):1921-1927,7.基金项目
南宁市科学研究与技术开发计划(201102048B) （201102048B）
