南方医科大学学报Issue(5)：636-640,5.DOI:10.3969/j.issn.1673-4254.2014.05.08
高龋学龄前儿童变形链球菌的水平传播方式
Horizontal transmission of Streptococcus mutans in caries-active preschool children
摘要
Abstract
Objective To analyze horizontal transmission patterns of Streptococcus mutans among caries-active preschool children for early interventions of dental caries. Methods Plaque samples obtained from 20 caries-active preschool children between 4 and 5 years of age were cultured under anaerobic conditions for isolating S. mutans, which were identified by morphological and biochemical analyses and PCR using primers homologous to the surface protein glucosyltransferase B (gtfB). The genotypes of the isolated S. mutans strains were determined by arbitrarily primed PCR (AP-PCR). Results Of the 200 S. mutans isolates obtained, 19 were excluded by biochemical analysis, and the remaining 181 isolates were identified as S. mutans by PCR with primers of gtfB, showing 37 different genotypes as identified by AP-PCR. Six children were found to carry S. mutans of a single genotype, 11 carried 2 genotypes, 2 had 3 genotypes, and 1 had 4 genotypes;2 children from different classes were found to carry S. mutans of the same single genotype. Conclusion We identified 37 genotypes of S. mutans in these caries-active preschool children, among whom horizontal transmissions of the strains were not found.关键词
学龄前儿童/变形链球菌/水平传播/基因型Key words
preschool children/Streptococcus mutans/horizontal transmission/genotypes引用本文复制引用
胡丹阳,崔伟,罗燕萍,杨继勇,邓斌,徐娟,储冰峰,王成龙..高龋学龄前儿童变形链球菌的水平传播方式[J].南方医科大学学报,2014,(5):636-640,5.基金项目
国家自然科学基金（81041028，81271191）@@@@Supported by National Natural Science Foundation of China (81041028,81271191) （81041028,81271191）
