南方医科大学学报Issue(6)：818-822,5.DOI:10.3969/j.issn.1673-4254.2014.06.13
CXCR7特异性拮抗剂SDF-1/54R的可溶性表达及活性评价
Soluble expression and activity evaluation of SDF-1/54R, a specific antagonist of CXCR7
摘要
Abstract
Objective To construct a soluble prokaryotic expression vector of the CXCR7-specific antagonist SDF-1/54R and evaluate its activity. Methods SDF-1/54r gene amplified by PCR was inserted into the soluble expression vector pET-41a+engineered with GST fusion tag, and the recombinant vector was transformed into E. coli strain BL21 (DE3). After IPTG induction of E. coli, the expressed recombinant protein was purified with GST affinity chromatography purification system and confirmed by SDS-PAGE and Western blotting assay. The target protein SDF-1/54R was obtained after digestion of the purified product with enterokinase. Breast cancer MCF-7 cells with high expression of CXCR7 was treated with SDF-1/54R and the cell proliferation and metastasis was evaluated with MTT and chemotaxis assays. Results The target protein SDF-1/54R obtained showed an obvious inhibitory effect on the proliferation and metastasis of MCF-7 cells as confirmed by MTT and chemotaxis assays. Conclusion SDF-1/54R is a good antagonist of CXCR7 and shows a potential value as an effective anti-cancer agent.关键词
CXCR7/拮抗剂/SDF-1/54R/可溶性表达Key words
CXCR7/antagonist/SDF-1/54R/soluble expression引用本文复制引用
曹园芝,杨飞华,马伟峰..CXCR7特异性拮抗剂SDF-1/54R的可溶性表达及活性评价[J].南方医科大学学报,2014,(6):818-822,5.基金项目
国家自然科学基金（81101732）；科技部重大新药创制计划（2012ZX09103-301-016）；教育部高等学校博士学科点专项科研基金（20104433120013）；广州市珠江科技新星专项基金（2013J2200047）；温州市科技计划（Y20100001）@@@@Supported byScience National Natural Foundation of China (81101732) （81101732）
