南方医科大学学报Issue(6)：827-831,5.DOI:10.3969/j.issn.1673-4254.2014.06.15
最佳剂量链脲佐菌素构建糖尿病小鼠的淋巴瘤模型
Optimization of streptozotocin dosing for establishing tumor-bearing diabetic mouse models
摘要
Abstract
Objective To determine the optimal dosing of streptozotocin (STZ) for establishing lymphoma-bearing diabetic mouse models. Methods A total of 200 healthy male Balb/c mice were randomized into 4 groups (n=50) for intraperitoneal injection of a single dose of vehicle solution (control) or 75, 150, or 200 mg/kg STZ. The changes of body weight and blood glucose were observed regularly, and the success rate of modeling, mortality rate, and survival of the mice were recorded after the injections. The mice with successfully induced diabetes received subcutaneous or tail vein injection of A20 lymphoma cells, and the rate of tumorigenesis, mortality rate, and survival time were observed at 1 month and 3 months after tumor cell injection. Results Compared with the control group, the mice receiving STZ injection at 150 and 200 mg/kg showed significantly decreased body weight and increased blood glucose (P<0.05), while STZ at 75 mg/kg did not produced such obvious changes. STZ injection at 200 mg/kg resulted in a significantly higher mortality rate and shorter survival time than STZ at 150 mg/kg (P<0.05). In the control group and 150 and 200 mg/kg STZ groups, the rate of tumorigenesis or mortality rate showed no significant differences after subcutaneous injection of A20 lymphoma cells (P>0.05), but differed significantly at 3 months after tail vein injection of the tumor cells (P<0.05). Conclusion Intraperitoneal injection of STZ at 150 mg/kg is associated with a low mortality rate, a high successful modeling rate of diabetes and a long survival time in mice, and is therefore optimal for establishing diabetic mouse models bearing transplanted tumors.关键词
链脲佐菌素/糖尿病/模型/Balb/c小鼠/淋巴瘤Key words
streptozotocin/diabetes/model/Balb/c mice/lymphoma引用本文复制引用
汤瑶,雷翔慧,简文静,严金海,吴自勍,赵彤..最佳剂量链脲佐菌素构建糖尿病小鼠的淋巴瘤模型[J].南方医科大学学报,2014,(6):827-831,5.基金项目
国家自然科学基金（81071659）@@@@Supported by National Natural Science Foundation of China (81071659) （81071659）
