南方医科大学学报Issue(6)：875-879,5.DOI:10.3969/j.issn.1673-4254.2014.06.24
京尼平交联的丝素蛋白-壳聚糖缓释微球的制备与表征
Preparation and characterization of genipin-crosslinked silk fibroin/chitosan controlled-release microspheres
摘要
Abstract
Objective To investigate the property of genipin-crosslinked silk fibroin(SF)/chitosan(CS) microspheres for slow releasing of bovine serum albumin (BSA). Methods BSA-loaded genipin-crosslinked SF/CS microspheres were prepared by emulsion cross-linking technique. The micropheres were observed for surface morphology and size distribution under scanning electron microscope (SEM), and X-ray diffractometry (XRD) and fourier transform infrared spectroscopy (FTIR) were used to analyze their structural characteristics. BCA method was used for determining the drug entrapment, loading rate and cumulative drug release in 21 days. Results The microspheres were spherical and showed a smooth surface with an average diameter of 7.84±0.97μm. The drug entrapment efficiency of the microspheres was (50.16±4.32)%with a drug loading ratio of (1.25 ± 0.11)% and a cumulative release of the total drug of (75.2 ± 2.53)% in 21 days. Conclusion Genipin-crosslinked SF/CS microspheres have a high drug entrapment efficiency and possess good capacity of sustained drug release.关键词
丝素蛋白/壳聚糖/京尼平/微球Key words
silk fibroin/chitosan/genipin/microspheres引用本文复制引用
叶漫文,曾曙光,高文峰,容明灯,郭泽鸿,石勇,干辉勇..京尼平交联的丝素蛋白-壳聚糖缓释微球的制备与表征[J].南方医科大学学报,2014,(6):875-879,5.基金项目
广东省科技计划社会发展项目（2011B031300022）；广东省卫生厅基金 （）
