南方医科大学学报Issue(7)：917-922,6.DOI:10.3969/j.issn.1673-4254.2014.07.01
曲古抑菌素对HepG2细胞增殖的影响及相关机制
Inhibitory effect of trichostatin A on HepG2 cell proliferation and the mechanisms
摘要
Abstract
Objective To investigate the inhibitory effect of trichostatin A (TSA) on the proliferation of HepG2 cells and explore the underlying mechanism. Methods HepG2 cells exposed to different concentrations of TSA for 24, 48, or 72 h were examined for cell growth inhibition using a cell counting kit, changes in cell cycle distribution with flow cytometry, cell apoptosis with annexin V-FTIC/PI double staining, and cell morphology changes under inverted microscope. The expressions of beta-catenin, HDAC1, HDAC3, H3K9, cyclinD1 and Bax proteins in the exposed cells were detected by Western blotting, and the expressions of HDAC1 and HDAC3 mRNAs by quantitative fluorescent PCR. Results Exposure to TSA caused significant dose-and time-dependent inhibition of HepG2 cell proliferation (P<0.05) and resulted in increased cell percentage in G0/G1 and G2/M phases and decreased cell percentage in S phase. The apoptotic index in the control group was (6.22 ± 0.25)%, which increased to (7.17 ± 0.20)%and (18.14 ± 0.42)%after exposure to 250 and 500 nmol/L TSA, respectively. Exposure to 250 and 500 nmol/L TSA also caused cell morphology changes with numerous floating cells. The expressions of beta-catenin, H3K9 and Bax proteins were significantly increased and CyclinD1, HDAC1, and HDAC3 protein expressions decreased in TSA-treated cells, but the expressions of HDAC1 and HDAC3 mRNAs showed no significant changes. Conclusion TSA can inhibit the proliferation of HepG2 cells and induce cell cycle arrest and apoptosis by inhibiting HDAC activity, promoting histone acetylation, and activating Wnt/beta-catenin signaling pathway.关键词
HepG2细胞/曲古抑菌素/凋亡/β-catenin/组蛋白乙酰化Key words
trichostatin A/HepG2 cells/apoptosis/beta-catenin/histone acetylation引用本文复制引用
石庆强,陈地龙,左国伟,冯子强,赵绿翠,罗念,游智梅,夏菁,李丹阳,李静..曲古抑菌素对HepG2细胞增殖的影响及相关机制[J].南方医科大学学报,2014,(7):917-922,6.基金项目
国家自然科学基金（31271368）；重庆市教委基金（KJ110308）@@@@Supported by National Natural Science Foundation of China (31271368) （31271368）
